IADR Abstract Archives
Association of polymorphisms to periodontitis in patients with down syndrome
Objectives: Down syndrome (DS) is one of the most common chromosomal conditions in humans, which manifests itself during cell division by presenting an additional copy on chromosome 21. In patients with DS there is a higher incidence of periodontal disease (PD) compared with the general population. PD includes a wide variety of chronic inflammatory diseases that affect the gingiva, bone, and periodontal ligament. Some studies suggest that cytokine polymorphisms could be associated with PD through a pro-inflammatory susceptibility.
Aim: To determine the presence of polymorphisms of the CCL5, CCR5, and TNF-α genes, and the levels of the cytokines IL-8, and IL-17 in patients with or without DS affected or not by PD.
Methods: A case-control study was carried out in the Diagnostic Department of the Faculty of Dentistry of the Autonomous University of Yucatan, and ethically approved (FODO-2019-0001). 24 patients with DS and with different degrees of PD were recruited, and patients without DS were selected as controls. A periodontal examination was performed on each participant to confirm the periodontal diagnosis. Saliva samples were obtained to determine the presence of the polymorphisms by qPCR, and the cytokines by ELISA. For the polymorphisms, the Hardy-Weinberg frequency and odds-ratio was determined. The cytokine levels were analyzed by ANOVA-Tukey.
Results: The GA and AA genotypes of the CCR5 G59029A polymorphism were a risk factor for PD, as well as the presence of the A allele. Also, higher levels of IL-8 and IL-17A were detected in patients with periodontitis stage III-IV.
Conclusions: The polymorphism of the CCR5 receptor G59029A could be considered as a risk factor in patients with DS to develop periodontitis stage III-IV. Otherwise, pro-inflammatory mediators correlate positively with polymorphisms and DS.
Aim: To determine the presence of polymorphisms of the CCL5, CCR5, and TNF-α genes, and the levels of the cytokines IL-8, and IL-17 in patients with or without DS affected or not by PD.
Methods: A case-control study was carried out in the Diagnostic Department of the Faculty of Dentistry of the Autonomous University of Yucatan, and ethically approved (FODO-2019-0001). 24 patients with DS and with different degrees of PD were recruited, and patients without DS were selected as controls. A periodontal examination was performed on each participant to confirm the periodontal diagnosis. Saliva samples were obtained to determine the presence of the polymorphisms by qPCR, and the cytokines by ELISA. For the polymorphisms, the Hardy-Weinberg frequency and odds-ratio was determined. The cytokine levels were analyzed by ANOVA-Tukey.
Results: The GA and AA genotypes of the CCR5 G59029A polymorphism were a risk factor for PD, as well as the presence of the A allele. Also, higher levels of IL-8 and IL-17A were detected in patients with periodontitis stage III-IV.
Conclusions: The polymorphism of the CCR5 receptor G59029A could be considered as a risk factor in patients with DS to develop periodontitis stage III-IV. Otherwise, pro-inflammatory mediators correlate positively with polymorphisms and DS.