IADR Abstract Archives

Identification of Cell Death-Associated and TLR-Regulator Proteins During Clinical Progression of Periodontitis

Objectives: Periodontitis is triggered by dysbiotic biofilms that elicit a local response with immuno-inflammatory dysregulation leading to a progressive tooth-support tissue destruction. There is no clear understanding of the cell death-associated and TLR-regulating molecular events occurring in the periodontal microenvironment during disease progression. Objective: To identify the presence of cell death-associated- and TLR-regulator proteins in the proteome of human GCF from progressive periodontal sites.
Methods: 16 periodontal patients were weekly monitored in their progression of periodontal destruction by clinical attachment loss and GCF samples were obtained. Three groups were established: Progression(PG); Pre-Progression(PP) and Non-Progression(NP). Proteins were processed using high-throughput proteomic approaches and label-free analysis determined their relative abundances. Proteins were identified at SwissProt database. Génie was searched for progression biomarkers. String and Gene Ontology Databases were searched for functional enrichment analysis and an integrated bioinformatics study of in silico and in vivo data was performed to determine proteins related to TLR-regulation, cell death and disease progression.
Results: 1572 proteins were identified: 1554, 1549 and 1550 in PP, PG and NP groups respectively. Proteomic characterization showed a higher abundance of IL1RN, IL36A and Phosphatases (PPP2CA, PTPN6 and MAP2K1) in NP group. A higher abundance of IL23R was found in PP group, while CA1, CA2 and several DAMPs (HSP90AA1, PRDX2, LGALS1, HEME, APOA1, NMI and SNCA) were more abundant in PG. The apoptotic-related proteins ANXA5 and H6PD were more abundant in NP vs. PG. The anti-ferroptosis-related protein FTH1 was exclusively identified in NP vs PG, and 4-fold increase in NP vs. PP; and the Ferroptosis-related protein GPX4 was more abundant in PG vs. NP.
Conclusions: There are quantitative and qualitative differences in the proteome of human GCF from periodontal sites. Different profiles of apoptosis-, ferroptosis-associated-, TLRs-regulator- and DAMPs proteins were identified according to the state of clinical progression of periodontitis. The understanding of their etiopathogenic role in progressive periodontitis may help to develop new diagnostic and therapeutic approaches.

2021 Chilean Division Annual Meeting (Virtual) 2021

2021

Periodontal Research-Pathogenesis
  • Torres, Alfredo  ( University of Chile , Santiago , Santiago , Chile ;  University of Chile , Santiago , santiago , Chile )
  • Lanyon, Elías  ( University of Chile , Santiago , Chile )
  • Pérez, Valentina  ( University of Chile , Santiago , Chile )
  • Arce, Marion  ( University of Chile , Santiago , Santiago , Chile )
  • Michea, Angélica  ( University of Chile , Santiago , Santiago , Chile )
  • Morales, Alicia  ( University of Chile , Santiago , Santiago , Chile )
  • Vegvari, Akos  ( Karolinska Institute , Stockholm , Sweden )
  • Zubarev, Roman  ( Karolinska Institute , Stockholm , Sweden )
  • González, Fermín  ( Universidad de Chile , Santiago , Chile ;  University of Chile , Santiago , santiago , Chile )
  • None
    Oral Session
    Temas Libres 3