Performance of a copper-containing adhesive in a cariogenic oral environment
Objectives: To evaluate the effect of the incorporation of copper nanoparticles (CuNp) into a universal adhesive on the antimicrobial activity (AMA), bond strength (µTBS), nanoleakage (NL), elastic modulus (EM) and nanohardness (NH) of the resin-dentin interfaces, at 24 h (24h) and after in situ cariogenic challenge (CC). Methods: CuNp (0% [control] and 0.1 wt%) were added in a universal adhesive. After enamel removal, the adhesives were applied to dentine surfaces. Each restored tooth was longitudinally sectioned to obtain two hemi-teeth; one of them was evaluated after 24h and the other was included in an intra-oral palatal device placed in the mouth of ten volunteers for 14 days in CC. After that, each hemi-tooth was removed and oral biofilm formed was collected. The AMA was evaluated against Streptococcus mutans. For 24h and CC groups, each hemi-tooth was sectioned in “x” direction to obtain one slice for each EM/NH evaluation. The remains of each hemi-tooth were sectioned in “x” and "y" direction to obtain resin-dentin beams for µTBS and NL evaluation (24h and CC). ANOVA and Tukey’s test were applied (α=0.05). Results: The presence of CuNp significantly improved AMA as well as all properties evaluated (24h; p<0.05). Although a decrease in the adhesive properties (µTBS/NL) for all groups after CC (p<0.05) occurred, the incorporation of CuNp maintained NH/EM values after CC (p<0.05). Conclusions: The addition of 0.1% CuNp in an adhesive may be an alternative to provide antimicrobial activity and increase its bonding and mechanical properties, even under a cariogenic challenge. This is the first in situ study proving that the incorporation of CuNp in a universal adhesive is a achievable alternative to provide antimicrobial properties and improved integrity of the hybrid layer under in situ cariogenic challenge.
Dental Materials 5: Biocompatibility, Bioengineering and Biologic Effects of Materials
Gutierrez, Felipe
( Universidad de los Andes
, Santiago
, Región Metropolitana
, Chile
; Universidad Finis Terrae
, Santiago
, Región Metropolitana
, Chile
)
Ñaupari-villasante, Romina
( State University of Ponta Grossa
, Ponta Grossa
, Paraná
, Brazil
)
De Paris Matos, Thalita
( State University of Ponta Grossa
, Ponta Grossa
, Paraná
, Brazil
)
Núñez, Alejandra
( State University of Ponta Grossa
, Ponta Grossa
, Paraná
, Brazil
)
Méndez-bauer, Luján
( State University of Ponta Grossa
, Ponta Grossa
, Paraná
, Brazil
)
Sutil, Elisama
( State University of Ponta Grossa
, Ponta Grossa
, Paraná
, Brazil
)
Loguercio, Alessandro
( State University of Ponta Grossa
, Ponta Grossa
, Paraná
, Brazil
)