IADR Abstract Archives

Molecular And Nanomechanical Characterization Of Honey-derived Exosomes Against Streptococcus mutans

Objectives: Recently, it has been shown that honey contains exosome-like extracellular vesicles (EVs) with strong antibacterial and antibiofilm effects against gram positive bacteria. The aim of our study was to characterize molecular and nanomechanical properties of EVs derived from A. mellifera honey (HEc-EVs) to get an idea of the possible mechanism of action of these EVs on gram positive bacterial strains.
Methods: A. mellifera honey-derived EVs from Eucryphia cordifolia (HEc-EVs) were isolated by ultracentrifugation and molecularly characterized with Western Blot and ELISA. To explore the nanomechanical and ultrastructural properties of HEc-EVs, atomic force microscopy (AFM) was employed.
Results: The characterization at the molecular level of the HEc-EVs allowed identification of the exosomal markers CD63 and syntenin. In addition, the antibacterial molecules MRJP1, defensin-1 and jellein-3 were found as intra-vesicular cargo. Nanomechanical results revealed that EVs were mostly <150nm, and low elastic modulus values comparable to EVs from other biological sources. AFM nanocharacterization showed alterations consistent with membrane damage on S. mutans when treated with EVs.
Conclusions: This is the first study reporting characterization of HEc-EVs exosomal markers and antibacterial peptides as cargo molecules. AFM nanocharacterization shows structural bacterial membrane alterations that explain potential mechanisms for bacterial viability inhibition when treated with HEc-EVs.

2021 Chilean Division Annual Meeting (Virtual) 2021


  • Leiva Sabadini, Camila  ( Pontificia Universidad Católica de Chile , Santiago , Chile )
  • Alvarez, Simon  ( Universidad del Desarrollo , Santiago , Chile )
  • Barrera, Nelson  ( Pontificia Universidad Católica de Chile , Santiago , Chile )
  • Schuh, Christina  ( Universidad del Desarrollo , Santiago , Chile )
  • Aguayo, Sebastian  ( Pontificia Universidad Catolica de Chile , Santiago , Chile )
  • NONE
    CONICYT FONDECYT 11180406 and 11180101
    Oral Session
    Temas Libres 2