IADR Abstract Archives
Remineralizing and antiproteolytic effect of fluoride / trimetaphosphate on dentin tissue
Objectives: the study evaluated the ability of solutions containing fluoride (F) and/or sodium trimetaphosphate (TMP) and F/TMP on the inhibition of MMP-2 and MMP-9, and on dentin remineralization in vitro.
Methods: Bovine root dentin blocks (6 × 4 × 2 mm, n = 130) were prepared, and caries-like lesions were induced in two thirds of the surface (each block served as its own control). Blocks were then were randomly divided into 13 groups/solutions: Placebo; 0.3%, 1% and 3% NaOH-hydrolyzed TMP; 0.3%, 1% and 3% TMP; 250 ppm F; 500 ppm F; 1100 ppm F; 250 ppm F + 0.3% TMP; 500 ppm F + 1% TMP and 1100 ppm F + 3% TMP. One third of each specimen was treated for one minute, twice a day with the respective solutions, and subjected to a pH-cycling regime for 7 days. The mineral concentration (gHAp × cm-3 × µm) was determined by computed X-ray microtomography, and data submitted to ANOVA and Student-Newman-Keuls’ test (p<0.05). The ability of the solutions on the inhibition of MMP-2 and MMP-9 activity was assessed by zymograpy.
Results: Subsurface lesions presented no mineral gain after treatment with F-free solutions (p<0.001). The mineral gain in the outermost region of the carious lesion showed dose-response relationship as a function of the F concentrations in the solutions (p<0.001), with no significant differences between their counterparts containing TMP (p>0.05). However, coadministration of F and TMP led to higher mineral gain in deeper regions of the lesion compared to counterparts without TMP (p<0.001). Solutions containing 3% TMP (hydrolyzed or not), 500 ppm F and 1100 ppm F completely inhibited MMP-2 activity, while for MMP-9 such effects were only achieved by treatment with 1100 ppm F + 3% TMP.
Conclusions: Treatment with 1100 ppm F + 3% TMP promoted the highest capacity to promote dentin remineralization, especially at deeper regions of the lesion, and to inhibit dentinal matrix metalloproteinases.
Methods: Bovine root dentin blocks (6 × 4 × 2 mm, n = 130) were prepared, and caries-like lesions were induced in two thirds of the surface (each block served as its own control). Blocks were then were randomly divided into 13 groups/solutions: Placebo; 0.3%, 1% and 3% NaOH-hydrolyzed TMP; 0.3%, 1% and 3% TMP; 250 ppm F; 500 ppm F; 1100 ppm F; 250 ppm F + 0.3% TMP; 500 ppm F + 1% TMP and 1100 ppm F + 3% TMP. One third of each specimen was treated for one minute, twice a day with the respective solutions, and subjected to a pH-cycling regime for 7 days. The mineral concentration (gHAp × cm-3 × µm) was determined by computed X-ray microtomography, and data submitted to ANOVA and Student-Newman-Keuls’ test (p<0.05). The ability of the solutions on the inhibition of MMP-2 and MMP-9 activity was assessed by zymograpy.
Results: Subsurface lesions presented no mineral gain after treatment with F-free solutions (p<0.001). The mineral gain in the outermost region of the carious lesion showed dose-response relationship as a function of the F concentrations in the solutions (p<0.001), with no significant differences between their counterparts containing TMP (p>0.05). However, coadministration of F and TMP led to higher mineral gain in deeper regions of the lesion compared to counterparts without TMP (p<0.001). Solutions containing 3% TMP (hydrolyzed or not), 500 ppm F and 1100 ppm F completely inhibited MMP-2 activity, while for MMP-9 such effects were only achieved by treatment with 1100 ppm F + 3% TMP.
Conclusions: Treatment with 1100 ppm F + 3% TMP promoted the highest capacity to promote dentin remineralization, especially at deeper regions of the lesion, and to inhibit dentinal matrix metalloproteinases.