IADR Abstract Archives
LPRF-plugs from smokers have the same tensile-strength properties as non-smokers
Objectives: In this study we want to analyze and to compare the tensile-strength properties of LPRF plugs from smokers and non-smokers.
Methods: 16 blood donors between 18 and 50 years old, 8 smokers and 8 non-smokers were included, after Pontificia Universidad Católica de Chile Scientific and Ethical Committee approval. All participants signed an informed consent. Briefly, venous blood samples were obtained in vacutainer tubes without anticoagulants and centrifuged 12 minutes at 406 X g. Then, LPRF clots were isolated from the middle of the tubes and compressed with a piston included in a stainless-steel device (X-pression-box) for 5 minutes to get plugs. Plugs were maintained in cell culture media until their analyses. Fresh LPRF plug samples, were evaluated in a universal Instron testing machine, using a quasi-static destructive tensile test at controlled temperature. Plugs were fixed using clamps at both while loading was applied. The Maximum load at sample failure were registered as a Stress-strain curve was stablished.
Results: In our in vitro study, the comparative mechanical parameters analyzed, evidenced that LPRF plugs from smokers have the same stiffness, elastic modulus, stretch length (non-smokers = 2,84 ± 0,28 [mm/mm], smokers = 2,69 ± 0,20 [mm/mm]) and the same resistance against tensile stress (non-smokers = 60,22 ± 14,64 [kPa], smokers = 46,17 ± 12,87 [kPa]).
Conclusions: With the limitations of our in vitro study, we can suggest that tensile-strength properties are not altered in LPRF plugs from smokers. More studies are necessary to characterize the effect of smoking habit in this plasmatic fraction as an alternative to promote wound healing in smokers.
Methods: 16 blood donors between 18 and 50 years old, 8 smokers and 8 non-smokers were included, after Pontificia Universidad Católica de Chile Scientific and Ethical Committee approval. All participants signed an informed consent. Briefly, venous blood samples were obtained in vacutainer tubes without anticoagulants and centrifuged 12 minutes at 406 X g. Then, LPRF clots were isolated from the middle of the tubes and compressed with a piston included in a stainless-steel device (X-pression-box) for 5 minutes to get plugs. Plugs were maintained in cell culture media until their analyses. Fresh LPRF plug samples, were evaluated in a universal Instron testing machine, using a quasi-static destructive tensile test at controlled temperature. Plugs were fixed using clamps at both while loading was applied. The Maximum load at sample failure were registered as a Stress-strain curve was stablished.
Results: In our in vitro study, the comparative mechanical parameters analyzed, evidenced that LPRF plugs from smokers have the same stiffness, elastic modulus, stretch length (non-smokers = 2,84 ± 0,28 [mm/mm], smokers = 2,69 ± 0,20 [mm/mm]) and the same resistance against tensile stress (non-smokers = 60,22 ± 14,64 [kPa], smokers = 46,17 ± 12,87 [kPa]).
Conclusions: With the limitations of our in vitro study, we can suggest that tensile-strength properties are not altered in LPRF plugs from smokers. More studies are necessary to characterize the effect of smoking habit in this plasmatic fraction as an alternative to promote wound healing in smokers.