IADR Abstract Archives
Helicobacter pylori and Porphyromonas gingivalis Interaction Enhance Cell Migration
Objectives: The objective of this work is to evaluate if the interaction between H. pylori and P. gingivalis increase the ability of P. gingivalis to promote migration in gingival epithelial cells. We also sought to determine if the presence of H. pylori induced changes in the expression of P. gingivalis genes associated with enhanced cell migration.
Methods: We defined conditions for the co-culture of H. pylori and P. gingivalis. For this, a liquid culture was standardized in which H. pylori 26695 and P. gingivalis W50 can grow and co-exist for 1 to 2 days (Brain-heart infusion, hemin, menadione, VITOX and equine serum, at 37 °C, in anaerobiosis and orbital agitation). After that P. gingivalis was isolated from the co-culture the ability to promote migration of OKF6-TERT2 cells was evaluated in transwell assays. RT-qPCR was performed to measure the mRNA levels of different gingipains of P. gingivalis relevant to cell migration (RgpA, RgpB and Kgp).
Results: Our results indicate that H. pylori increases the ability of P. gingivalis to promote migration of OKF6-TERT2 gingival epithelial cells compared to P. gingivalis grown in monoculture. Also, co-culturing increased the expression of the P. gingivalis gene rgpB, which is known to promote the migration of infected cells.
Conclusions: A synergistic interaction between H. pylori and P. gingivalis was observed, in which H. pylori enhanced the ability of P. gingivalis to promote gingival epithelial cell migration, thereby contributing to alterations in gingival epithelial organization. Moreover, our results suggest that H. pylori enhances the ability of P. gingivalis to promote cell migration by increasing the expression of bacterial gingipains.
Methods: We defined conditions for the co-culture of H. pylori and P. gingivalis. For this, a liquid culture was standardized in which H. pylori 26695 and P. gingivalis W50 can grow and co-exist for 1 to 2 days (Brain-heart infusion, hemin, menadione, VITOX and equine serum, at 37 °C, in anaerobiosis and orbital agitation). After that P. gingivalis was isolated from the co-culture the ability to promote migration of OKF6-TERT2 cells was evaluated in transwell assays. RT-qPCR was performed to measure the mRNA levels of different gingipains of P. gingivalis relevant to cell migration (RgpA, RgpB and Kgp).
Results: Our results indicate that H. pylori increases the ability of P. gingivalis to promote migration of OKF6-TERT2 gingival epithelial cells compared to P. gingivalis grown in monoculture. Also, co-culturing increased the expression of the P. gingivalis gene rgpB, which is known to promote the migration of infected cells.
Conclusions: A synergistic interaction between H. pylori and P. gingivalis was observed, in which H. pylori enhanced the ability of P. gingivalis to promote gingival epithelial cell migration, thereby contributing to alterations in gingival epithelial organization. Moreover, our results suggest that H. pylori enhances the ability of P. gingivalis to promote cell migration by increasing the expression of bacterial gingipains.