IADR Abstract Archives
EXPRESSION OF BRCA1 PROTEIN IN DIAGNOSED BIOPSIES WITH ORAL EPITHELIAL DYSPLASIA AND ORAL SQUAMOUS CELL CARCINOMA
Objectives: BRCA1 is a tumor suppressor gene that is part of the DNA detection and repair system. It has been used as an important biomarker for diagnosis and prognostic evaluation in various carcinomas; however, oral cancer research is still in its beginning. The purpose of this study was to characterize the protein expression of BRCA1 in samples of DEO and COCE, and associate them in addition to variables such as age and gender.
Methods: A total of 45 patients (20 DEO, 20 COCE and 5 FI) were studied, whose samples were obtained from the Diagnostic and Emergency Service of the Universidad Mayor’s School of Dentistry. They were analyzed by immunohistochemical technique and the cell count was performed using the ImageJ Software. The expression was compared in terms of staining, expression pattern, percentage of positive cells and the index of marking; and these variables were related to the degree of cell differentiation of the samples, histological layer of expression, in addition to being associated with clinical variables such as age of diagnosis and gender.
Results: Differences were obtained between the protein expression of BRCA1 in DEO and COCE, specifically regarding the marking index of them; likewise, the existence of a down-regulation of expression was observed in the transition from DEO to COCE, to later generate an increase in protein expression. In the normal state of expression, BRCA1 was found in the basal and parabasal layers of the tissues with a low intensity of expression, and then during the carcinogenesis process, it changes towards an intermediate and lucid layer. Aberrant locations of expression were observed, both in cytoplasm and membrane, which may be related to the migration of cancer cells and their potential for malignancy. In addition, a trend was observed in relation to the male gender ratio in DEO and BRCA1 expression; and no relationship regarding protein expression and age of diagnosis of pathologies
Conclusions: Finally, it can be concluded that the protein expression of BRCA1 does differ according to the degree of tissue differentiation, and that the aberrant location of expression may be a future therapeutic strategy in these lesions
Methods: A total of 45 patients (20 DEO, 20 COCE and 5 FI) were studied, whose samples were obtained from the Diagnostic and Emergency Service of the Universidad Mayor’s School of Dentistry. They were analyzed by immunohistochemical technique and the cell count was performed using the ImageJ Software. The expression was compared in terms of staining, expression pattern, percentage of positive cells and the index of marking; and these variables were related to the degree of cell differentiation of the samples, histological layer of expression, in addition to being associated with clinical variables such as age of diagnosis and gender.
Results: Differences were obtained between the protein expression of BRCA1 in DEO and COCE, specifically regarding the marking index of them; likewise, the existence of a down-regulation of expression was observed in the transition from DEO to COCE, to later generate an increase in protein expression. In the normal state of expression, BRCA1 was found in the basal and parabasal layers of the tissues with a low intensity of expression, and then during the carcinogenesis process, it changes towards an intermediate and lucid layer. Aberrant locations of expression were observed, both in cytoplasm and membrane, which may be related to the migration of cancer cells and their potential for malignancy. In addition, a trend was observed in relation to the male gender ratio in DEO and BRCA1 expression; and no relationship regarding protein expression and age of diagnosis of pathologies
Conclusions: Finally, it can be concluded that the protein expression of BRCA1 does differ according to the degree of tissue differentiation, and that the aberrant location of expression may be a future therapeutic strategy in these lesions