IADR Abstract Archives
Expression of Angiogenic Factors in Symptomatic and Asymptomatic Apical Periodontitis
Objectives: To profile the angiogenesis related proteins in symptomatic apical periodontitis (SAP), asymptomatic apical periodontitis (AAP) and healthy controls.
Methods: Patients consulting at the dental clinic of the Faculty of Dentistry with diagnosis of SAP, AAP and healthy control were included (N=5). Samples of apical lesions and healthy periodontal ligament as control were collected. The specimens were homogenized and angiogenic factors were measured using a commercial profiler with semi-quantitative assessing of 55 proteins on nitrocellulose membranes and densitometric scanning.
Results: Seven proteins showed evidenced variations among studied groups. A decrease in Serpin-1 was observed in AAP and SAP compared to the control group. MMP-9 and MMP-8 levels increased in AAP. PTX3, ET-1, Ang-2, DPP-IV were lower in SAP, higher in AAP and the highest in control group.
Conclusions: Differential angiogenesis related protein profiles were observed in SAP, AAP and controls, supporting a role of angiogenesis in AP evolution /exacerbation.
Methods: Patients consulting at the dental clinic of the Faculty of Dentistry with diagnosis of SAP, AAP and healthy control were included (N=5). Samples of apical lesions and healthy periodontal ligament as control were collected. The specimens were homogenized and angiogenic factors were measured using a commercial profiler with semi-quantitative assessing of 55 proteins on nitrocellulose membranes and densitometric scanning.
Results: Seven proteins showed evidenced variations among studied groups. A decrease in Serpin-1 was observed in AAP and SAP compared to the control group. MMP-9 and MMP-8 levels increased in AAP. PTX3, ET-1, Ang-2, DPP-IV were lower in SAP, higher in AAP and the highest in control group.
Conclusions: Differential angiogenesis related protein profiles were observed in SAP, AAP and controls, supporting a role of angiogenesis in AP evolution /exacerbation.