IADR Abstract Archives
Collagen Quantification In Peri-Implant Soft Tissues Of Human Peri-Implant Lesions
Objectives: To quantify the percentage of collagen fibers present in the peri-implant soft tissue of patients with peri-implantitis.
Methods: Descriptive observational cross-sectional study. Eleven peri-implant soft tissue samples were collected from patients with peri-implantitis at the Peri-implantology Polyclinic of the Faculty of Dentistry of the Universidad de La Frontera, Temuco, Chile. Ethical approval to carry out the study was granted by the Ethics Committee of the Universidad de la Frontera, Report N°. 024_2018 (REDI Project 0170658, CONICYT). After the written informed consent, the biopsies have undergone a histopathological analysis at the Oral Pathology Laboratory of the University of Talca. All samples were processed using Mason's Trichomic Technique. The reading and interpretation of results and image capture were performed under 40X magnification light microscopy with a camera (Canon EOS Rebel XSI, Tokyo, Japan). 5 fields per sheet were analyzed. Quantification of collagen fibers was performed using Image J software (version 1.46j; National Institute of Health, USA) with application of deconvolution color. Collagen density is expressed as a percentage of the estimated total area. The data is grouped to represent a mean value and standard deviation, determining minimum and maximum values.
Results: Macroscopic collagen fiber bundles were calculated in all samples, with an average percentage (38.69%), standard deviation (16.64%), with a minimum value (8.99%), and a maximum value (69.91%) density.
Conclusions: This study showed that in human peri-implantitis lesions there is a moderate percentage of collagen fibers (38.69%) in peri-implant soft tissue. This is interpreted as connective tissue in a permanent process of reparative response, in the presence of an inflammatory infiltrate. This phenomenon should be investigated in depth in future studies with a larger sample size, and compared to healthy peri-implant soft tissue samples, however it serves as a preliminary result for future research.
Methods: Descriptive observational cross-sectional study. Eleven peri-implant soft tissue samples were collected from patients with peri-implantitis at the Peri-implantology Polyclinic of the Faculty of Dentistry of the Universidad de La Frontera, Temuco, Chile. Ethical approval to carry out the study was granted by the Ethics Committee of the Universidad de la Frontera, Report N°. 024_2018 (REDI Project 0170658, CONICYT). After the written informed consent, the biopsies have undergone a histopathological analysis at the Oral Pathology Laboratory of the University of Talca. All samples were processed using Mason's Trichomic Technique. The reading and interpretation of results and image capture were performed under 40X magnification light microscopy with a camera (Canon EOS Rebel XSI, Tokyo, Japan). 5 fields per sheet were analyzed. Quantification of collagen fibers was performed using Image J software (version 1.46j; National Institute of Health, USA) with application of deconvolution color. Collagen density is expressed as a percentage of the estimated total area. The data is grouped to represent a mean value and standard deviation, determining minimum and maximum values.
Results: Macroscopic collagen fiber bundles were calculated in all samples, with an average percentage (38.69%), standard deviation (16.64%), with a minimum value (8.99%), and a maximum value (69.91%) density.
Conclusions: This study showed that in human peri-implantitis lesions there is a moderate percentage of collagen fibers (38.69%) in peri-implant soft tissue. This is interpreted as connective tissue in a permanent process of reparative response, in the presence of an inflammatory infiltrate. This phenomenon should be investigated in depth in future studies with a larger sample size, and compared to healthy peri-implant soft tissue samples, however it serves as a preliminary result for future research.
3468249_File000002.jpg
3468249_File000003.jpg