IADR Abstract Archives

Antimicrobial action of NeoMTA Plus: an in vitro study

Objectives: The aim of the present study was to evaluate the antimicrobial capacity of NeoMTA Plus (Avalon Biomed, Houston, TX, USA) on single and mixed biofilms of Enterococcus faecalis and Candida albicans.
Methods: A total of 171 sterile dentin blocks, measuring 4 × 4 × 1 mm, were incubated in medium containing E. faecalis (3.1×108 cells/mL) and/or C. albicans (1×107 cells/mL) for 2 days. These blocks were randomly divided into three groups: control (no treatment with biomaterials); MTA, treated with a sample of MTA after setting, with the same width and thickness of the dentin block; and NEO, similar to the MTA group, but treated with NeoMTA Plus. The biomaterials remained in contact with the biofilms for 24 h. Quantitative analyses, including the number of colony-forming units (CFUs) and metabolic activity (XTT), were performed. Furthermore, qualitative analysis of biofilm structure was performed using scanning electron microscopy. Data were analyzed and subjected to statistical analysis using a significance level of 5% (p<0.05).
Results: XTT and the number of CFUs were similar among the groups (p>0.05). The type of biofilm (i.e., single or mixed) or biomaterial used (i.e., MTA or NeoMTA Plus) did not affect the results. The structure of the biofilms remained homogeneous among the groups.
Conclusions: NeoMTA Plus was not effective against single and mixed biofilms of E. faecalis and C. albicans. Further research investigating methods to remove biofilms is warranted, including the use of these biomaterials with antiseptics and supporting therapies.

2020 Chilean Division Meeting (Virtual)

2020

Microbiology/Immunology
  • Martins, Christine  ( Universidade do Oeste Paulista , Maringá , Brazil )
  • Jacob, Vanessa  ( Universidade do Oeste Paulista , Maringá , Brazil )
  • Paião, Luana  ( Universidade do Oeste Paulista , Maringá , Brazil )
  • Da Silva, Ana Carolina  ( Universidade do Oeste Paulista , Maringá , Brazil )
  • Magario, Mychelle  ( Universidade do Oeste Paulista , Maringá , Brazil )
  • Kaneko, Taís  ( Universidade do Oeste Paulista , Maringá , Brazil )
  • Monteiro, Douglas  ( Universidade do Oeste Paulista , Maringá , Brazil )
  • Mori, Graziela  ( Universidade do Oeste Paulista , Maringá , Brazil )
  • NONE
    Poster Session
    Poster Session B