IADR Abstract Archives
Subgingival Irrigation with Phytotherapics Adjunct to Scaling and Root Planing
Objectives: This study evaluated influence of subgingival irrigation with Matricaria recutita (MAT) and Plantago major (PLA) adjunct to scaling and root planing (SRP) on treatment of experimental periodontitis (EP) in rats.
Methods: EP was induced in 72 rats with a ligature around the mandibular left first molar. After 7 days, the ligature was removed, and the animals were randomly distributed into 3 groups: SRP – SRP and irrigation with saline; MAT - SRP and irrigation with MAT solution; and PLA - SRP and irrigation with PLA solution. Each group was subdivided into 3 subgroups (n = 8) and euthanasia performed after 7, 15 and 30 days. It was evaluated colony-forming units (CFU), area of bone loss (BL) in the furcation region, percentage of mature and immature collagen fibers, tartrate-resistant acid phosphatase (TRAP), ligand of the nuclear factor kappa B activator receptor (RANKL) and osteoprotegerin (OPG). Data were statistically analyzed (p<0.05).
Results: Groups MAT and PLA had significantly lower number of CFU than Group SRP within 15 days. Group PLA had significantly lower BL than Group MAT 7 days. Group MAT had significantly higher percentage of immature collagen fibers than groups SRP and PLA 15 days. Group PLA presented significantly higher OPG than Group SRP 7 days and significantly lower RANKL than groups MAT and SRP 15 days.
Conclusions: It can be concluded that subgingival irrigation with Matricaria recutita and Plantago major adjunct to SRP reduces the amount of CFU and improves periodontal healing in the treatment of EP in rats.
Methods: EP was induced in 72 rats with a ligature around the mandibular left first molar. After 7 days, the ligature was removed, and the animals were randomly distributed into 3 groups: SRP – SRP and irrigation with saline; MAT - SRP and irrigation with MAT solution; and PLA - SRP and irrigation with PLA solution. Each group was subdivided into 3 subgroups (n = 8) and euthanasia performed after 7, 15 and 30 days. It was evaluated colony-forming units (CFU), area of bone loss (BL) in the furcation region, percentage of mature and immature collagen fibers, tartrate-resistant acid phosphatase (TRAP), ligand of the nuclear factor kappa B activator receptor (RANKL) and osteoprotegerin (OPG). Data were statistically analyzed (p<0.05).
Results: Groups MAT and PLA had significantly lower number of CFU than Group SRP within 15 days. Group PLA had significantly lower BL than Group MAT 7 days. Group MAT had significantly higher percentage of immature collagen fibers than groups SRP and PLA 15 days. Group PLA presented significantly higher OPG than Group SRP 7 days and significantly lower RANKL than groups MAT and SRP 15 days.
Conclusions: It can be concluded that subgingival irrigation with Matricaria recutita and Plantago major adjunct to SRP reduces the amount of CFU and improves periodontal healing in the treatment of EP in rats.