IADR Abstract Archives
In vitro evaluation of the association of toothpaste and mouthrinse on dental enamel demineralization
Objectives: The aim of this study was to evaluate in vitro capacity of the association of media between fluoridated toothpastes and mouthrinses supplemented or not with sodium trimetophosphate (TMP), in reduzing dental enamel demineralization.
Methods: Bovine blocks (n=60) were selected for their initial surface hardness (SHi) and then divided into 5 experimental groups (n=12): 1) Toothpaste without F/TMP (Placebo); 2) Toothpaste 1100 ppm F (1100F), 3) Toothpaste 1100F associated with a mouthrinse with 100 ppm F (1100F-100F), 4) Toothpaste 1100F associated with a mouthrinse with 225 ppm F (1100F-225F) and 5) 1100F toothpaste associated with a 100 ppm F mouthrinse supplemented with 0.4% TMP (1100F-100F-TMP). The blocks were treated with 2 mL of toothpaste slurry and mouthrinses 2x/day, being subjected to 5 pH cycles for 7 days. After pH cycling, the final surface hardness (SHf) was determined to calculate the percentage of loss of surface hardness (%SH). The data were submitted to a variance analysis (ANOVA-1-criterion) followed by the Student-Newman-Keuls test (p < 0.001).
Results: For %SH, the 1100F-100F-TMP group reduced enamel demineralization by ~ 15% compared to treatment with 1100F (p <0.001), being similar to the 1100F-225F (p > 0.001). Blocks treated with 1100F only presented the highest %SH when compared to the other treatments (1100F-100F; 1100F-225F; 1100F-100F-TMP) (p > 0.001).
Conclusions: It was concluded that the toothpaste and mouthrinse association promoted a greater inhibitory effect against enamel demineralization when compared to toothpaste, and that the TMP supplementation in the 100F mouthrinse obtained a similar effect to a 225F mouthrinse. Clinical Significance: The combined treatments may be an alternative for patients at high risk of dental caries.
Methods: Bovine blocks (n=60) were selected for their initial surface hardness (SHi) and then divided into 5 experimental groups (n=12): 1) Toothpaste without F/TMP (Placebo); 2) Toothpaste 1100 ppm F (1100F), 3) Toothpaste 1100F associated with a mouthrinse with 100 ppm F (1100F-100F), 4) Toothpaste 1100F associated with a mouthrinse with 225 ppm F (1100F-225F) and 5) 1100F toothpaste associated with a 100 ppm F mouthrinse supplemented with 0.4% TMP (1100F-100F-TMP). The blocks were treated with 2 mL of toothpaste slurry and mouthrinses 2x/day, being subjected to 5 pH cycles for 7 days. After pH cycling, the final surface hardness (SHf) was determined to calculate the percentage of loss of surface hardness (%SH). The data were submitted to a variance analysis (ANOVA-1-criterion) followed by the Student-Newman-Keuls test (p < 0.001).
Results: For %SH, the 1100F-100F-TMP group reduced enamel demineralization by ~ 15% compared to treatment with 1100F (p <0.001), being similar to the 1100F-225F (p > 0.001). Blocks treated with 1100F only presented the highest %SH when compared to the other treatments (1100F-100F; 1100F-225F; 1100F-100F-TMP) (p > 0.001).
Conclusions: It was concluded that the toothpaste and mouthrinse association promoted a greater inhibitory effect against enamel demineralization when compared to toothpaste, and that the TMP supplementation in the 100F mouthrinse obtained a similar effect to a 225F mouthrinse. Clinical Significance: The combined treatments may be an alternative for patients at high risk of dental caries.