IADR Abstract Archives
Protease Profile In Oral Fluid Of Individuals With Moderate/Severe Atopic Dermatitis
Objectives: To explore the protease profile in the gingival crevicular fluid (GCF) of individuals with moderate/severe AD compared with healthy controls.
Methods: A case control study was conducted. AD patients (n=6) and controls (n=6) were selected at the International Center for Clinical Studies in Santiago-Chile. A complete dermatological and periodontal evaluation that involved the collection of GCF was performed at the same gender and age of both groups. The levels of 35 human proteases in GCF were analyzed using a human protease antibody array and semi-quantified by densitometric analysis.
Results: Thirty five proteases were detected in the GCF of moderate/severe AD patients and controls. The levels of ADAM8, ADAM9, Cathepsin E, MMP8, Neprilysin/CD10, Protein convertase9 and uPA/Urokinase in GCF were lower in moderate/severe AD patients than controls (p<0.05). No inter-group differences in the levels of the other 28 proteases were detected.
Conclusions: ADAM8, ADAM9, Cathepsin E, MMP8, CD10, Protein convertase9 and uPA/Urokinase proteases were down-regulated in the GCF of moderate/severe atopic dermatitis patients versus controls. The combination of these proteases may be useful biomarkers for the detection of the severity of the disease.
Methods: A case control study was conducted. AD patients (n=6) and controls (n=6) were selected at the International Center for Clinical Studies in Santiago-Chile. A complete dermatological and periodontal evaluation that involved the collection of GCF was performed at the same gender and age of both groups. The levels of 35 human proteases in GCF were analyzed using a human protease antibody array and semi-quantified by densitometric analysis.
Results: Thirty five proteases were detected in the GCF of moderate/severe AD patients and controls. The levels of ADAM8, ADAM9, Cathepsin E, MMP8, Neprilysin/CD10, Protein convertase9 and uPA/Urokinase in GCF were lower in moderate/severe AD patients than controls (p<0.05). No inter-group differences in the levels of the other 28 proteases were detected.
Conclusions: ADAM8, ADAM9, Cathepsin E, MMP8, CD10, Protein convertase9 and uPA/Urokinase proteases were down-regulated in the GCF of moderate/severe atopic dermatitis patients versus controls. The combination of these proteases may be useful biomarkers for the detection of the severity of the disease.