IADR Abstract Archives

Translocation of Endodontic Pathogens through Mononuclear Blood Cells

Objectives: We aimed to first explore overall extra-radicular infection as well as by specific Porphyromonas (P). spp; and their potential to translocate from infected root canals to blood through peripheral blood mononuclear cells (PBMCs).
Methods: In this cross-sectional study, healthy individuals with and without apical periodontitis (AP) diagnosis with an associated apical lesion of endodontic origin (ALEO) were included. ALEOs (n=64) were obtained from volunteers with asymptomatic apical periodontitis (AAP; n=29) and symptomatic apical periodontitis (SAP; n=35). Intracanal samples (n=39; AAP=29, SAP=10), their respective PBMCs and controls (n=14/per group) were included. Total bacteria load, as well as P. endodontalis and P. gingivalis were measured by qPCR.
Results: In ALEOs, the detection frequency of total bacteria, P. endodontalis and P. gingivalis (bacterial load [median DNA copy number/mg]) were 70.8% (4521.6), 21.5% (1789.7), 18.4% (1493.9), respectively (p>0.05). In intracanal exudates, the total bacterial detection (load [median DNA copy number/μL]) was 100% (21089.2); P. endodontalis and P. gingivalis were detected in 41% (8263.9) and 20.5% (12538.9) of the samples, respectively. In PBMCs, total bacteria was detected in all samples; the bacterial load (median DNA copy number/μL) was significantly higher in AP (953.6) compared to controls (300.7; p<0.05). P. endodontalisdetection was equal for both groups (50%), but bacterial load tended to be higher in AP (262.3) than controls (158.8; p>0.05); P. gingivalis was not detected.
Conclusions: DNA from the specific endodontic pathogen P. endodontalis was detected in endodontic canals, ALEOs and in PMBC. Higher bacterial loads can be identified in PMBC from AP compared to healthy individuals. PMBC might carry endodontic bacteria/DNA to distant tissue/sites.

2020 Chilean Division Meeting (Virtual)

2020

Oral Medicine & Pathology Research
  • Fernandez, Alejandra  ( Universidad Andres Bello , Santiago , Chile ;  Universidad de Chile , Santiago de Chile , Chile )
  • Bordagaray, María José  ( Universidad de Chile , Santiago de Chile , Chile )
  • Garrido, Mauricio  ( Universidad de Chile , Santiago de Chile , Chile )
  • Hoare, Anilei  ( Universidad de Chile , Santiago de Chile , Chile )
  • Astorga, Jessica  ( Universidad de Chile , Santiago de Chile , Chile )
  • Hernández, Marcela  ( Universidad de Chile , Santiago de Chile , Chile )
  • NONE
    Funding by FONDECYT 1160741 and 1200098
    Oral Session
    Oral Session 4