IADR Abstract Archives
Ionic Changes in Biofilms Treated with Polyphosphate, Polyols and Fluoride
Objectives: This study assessed the effect of sodium trimetaphosphate (TMP), xylitol (X), erythritol (E) and fluoride (F), alone or in association, on F, calcium (Ca) and phosphorus (Pi) concentrations in mixed biofilms of S. mutans and C. albicans formed in vitro.
Methods: Biofilms were formed in culture media containing TMP (0.025%), X (0.16%), E (0.04%), 20 ppm F (20F), alone or associated as X+E, TMP+X+E, 20F+X+E, 20F+TMP, and 20F+TMP+X+E (experimental). Culture medium containing 110 ppm F and pure culture medium were used as positive and negative controls, respectively. Biofilms (96 h) were collected both before and after exposure to 20% sucrose solution, and were analyzed regarding F (ion-specific electrode), Ca (Arsenazo III) and Pi (molybdate method) concentrations, in the solid and fluid phases of the biofilms. Data were submitted to bidirectional analysis of variance, followed by Fisher's LSD test (p <0.05).
Results: F concentrations were significantly increased in the biofilm fluid of the experimental group compared with 20F. Also, the experimental group had the highest Ca concentrations in the biomass prior to exposure to sucrose, and increased the Ca concentration in the biofilm fluid after exposure to sucrose. The groups treated with TMP presented the highest values of Pi in the biofilm fluid. Overall, all inorganic components analyzed reduced after sucrose exposure, except for the Ca concentration in the fluid phase in the biofilm.
Conclusions: It was concluded that simultaneous exposure to X, E, F and TMP (experimental group) increases the F levels in the biofilm fluid and affects Ca concentrations in the solid and fluid phases of the biofilms, before and after sucrose exposure.
Methods: Biofilms were formed in culture media containing TMP (0.025%), X (0.16%), E (0.04%), 20 ppm F (20F), alone or associated as X+E, TMP+X+E, 20F+X+E, 20F+TMP, and 20F+TMP+X+E (experimental). Culture medium containing 110 ppm F and pure culture medium were used as positive and negative controls, respectively. Biofilms (96 h) were collected both before and after exposure to 20% sucrose solution, and were analyzed regarding F (ion-specific electrode), Ca (Arsenazo III) and Pi (molybdate method) concentrations, in the solid and fluid phases of the biofilms. Data were submitted to bidirectional analysis of variance, followed by Fisher's LSD test (p <0.05).
Results: F concentrations were significantly increased in the biofilm fluid of the experimental group compared with 20F. Also, the experimental group had the highest Ca concentrations in the biomass prior to exposure to sucrose, and increased the Ca concentration in the biofilm fluid after exposure to sucrose. The groups treated with TMP presented the highest values of Pi in the biofilm fluid. Overall, all inorganic components analyzed reduced after sucrose exposure, except for the Ca concentration in the fluid phase in the biofilm.
Conclusions: It was concluded that simultaneous exposure to X, E, F and TMP (experimental group) increases the F levels in the biofilm fluid and affects Ca concentrations in the solid and fluid phases of the biofilms, before and after sucrose exposure.