IADR Abstract Archives
Human Amniotic Ephitelial Cells: Mineralization Capacity and Adhesion to Dentin
Objectives: The aim of this study was to evaluate the mineralization capacity of human ammiotic epithelial cells (hAECs) after being cultured with dentin matrix proteins (DMPs) or an osteogenic differentiation medium (StemPro). The adherence and adoption of odontoblast-like phenotype of hAECs after being seeded onto dentin disks treated with EDTA was also evaluated.
Methods: hAECs were isolated from human placentas and the purity of the isolated cells was evaluated through flow cytometry. DMPs were extracted from third molars. Dental pulp stem cells (DPSCs) were used as control group. To evaluate mineralization capacity, the cells were cultured in DMEM with 500 pg/ml DMPs or the osteogenic differentiation medium, and calcium deposits were assessed by the alizarin red staining method (day 21). To evaluate adhesion to dentin, cells were seeded onto dentin disks previously treated with EDTA and evaluated trough ESEM after 48 hours.
Results: hAECs were able to secrete calcium deposits, although to a lesser extent compared to DPSCs.
The ESEM images of both cell types showed adhesion to and spreading onto the dentin surface. Both hAECs and DPSCs exhibit extended processes onto the dentin surface, some of them located into the dentin tubuli, and cell–cell contacts.
Conclusions: Human amniotic ephitelial cells are able to mineralize and adhere to dentin.
Methods: hAECs were isolated from human placentas and the purity of the isolated cells was evaluated through flow cytometry. DMPs were extracted from third molars. Dental pulp stem cells (DPSCs) were used as control group. To evaluate mineralization capacity, the cells were cultured in DMEM with 500 pg/ml DMPs or the osteogenic differentiation medium, and calcium deposits were assessed by the alizarin red staining method (day 21). To evaluate adhesion to dentin, cells were seeded onto dentin disks previously treated with EDTA and evaluated trough ESEM after 48 hours.
Results: hAECs were able to secrete calcium deposits, although to a lesser extent compared to DPSCs.
The ESEM images of both cell types showed adhesion to and spreading onto the dentin surface. Both hAECs and DPSCs exhibit extended processes onto the dentin surface, some of them located into the dentin tubuli, and cell–cell contacts.
Conclusions: Human amniotic ephitelial cells are able to mineralize and adhere to dentin.