IADR Abstract Archives
Histopathological findings in peri-implant tissue from patients with peri-implantitis.
Objectives: To determine the structural histological characteristics of peri-implant tissue in explanted samples from patients with dental implants with osseointegration failure. It is also proposed to analyze a probable association with clinical variables.
Methods: Fourteen peri-implant tissue samples were processed by conventional histological technique, stained with Hematoxylin and Eosin, histochemical and immunohistochemical stains. Microvascular Density, inflammatory infiltrate, connective tissue and AgNORs were qualitatively analyzed using a Leica DM 500 microscope and Leica ICC50 W camera and software Image J 1.52.The study is carried out in the context of the research project "Immunohistochemical, histochemical and endoscopic analysis of bone tissue associated with periimplantitis: Development of an In vivo and In vitro model", awarded in the Conicyt competition to support the formation of international networks for researchers in initial stage 2017, whose code is “REDI170658”. Ethics approval by University of La Frontera, Chile.
Results: Variable amounts of blood vessels and inflammatory infiltrate were found in soft periimplant tissues, evidencing presence of periimplant mucositis. Inflammatory cells were mainly lymphocytes and plasma cells, which means presence of a chronical stage of inflammation. No foreign body reaction was found and scarce amount of osteoclasts were observed in periimplant bone tissue. Mainly collagen type one was found in connective tissue, which is interpreted as a normal process of healing associated to inflammation. Number of AgNORs were observed in increased number in epithelial cells near inflammatory process. No association to clinical variables could be observed.
Conclusions: Histopathological findings observes in periimplant soft and bone tissue are according with a common chronical inflammatory process. Possible associations with clinical characteristics require further analysis.
Methods: Fourteen peri-implant tissue samples were processed by conventional histological technique, stained with Hematoxylin and Eosin, histochemical and immunohistochemical stains. Microvascular Density, inflammatory infiltrate, connective tissue and AgNORs were qualitatively analyzed using a Leica DM 500 microscope and Leica ICC50 W camera and software Image J 1.52.The study is carried out in the context of the research project "Immunohistochemical, histochemical and endoscopic analysis of bone tissue associated with periimplantitis: Development of an In vivo and In vitro model", awarded in the Conicyt competition to support the formation of international networks for researchers in initial stage 2017, whose code is “REDI170658”. Ethics approval by University of La Frontera, Chile.
Results: Variable amounts of blood vessels and inflammatory infiltrate were found in soft periimplant tissues, evidencing presence of periimplant mucositis. Inflammatory cells were mainly lymphocytes and plasma cells, which means presence of a chronical stage of inflammation. No foreign body reaction was found and scarce amount of osteoclasts were observed in periimplant bone tissue. Mainly collagen type one was found in connective tissue, which is interpreted as a normal process of healing associated to inflammation. Number of AgNORs were observed in increased number in epithelial cells near inflammatory process. No association to clinical variables could be observed.
Conclusions: Histopathological findings observes in periimplant soft and bone tissue are according with a common chronical inflammatory process. Possible associations with clinical characteristics require further analysis.