IADR Abstract Archives
Can Porphyromonas gingivalis migrate from periodontal tissues towards brain?
Objectives: Porphyromonas gingivalis is a Gram negative bacteria widely associated with aetiopathogenia of periodontitis, and the different P. gingivalis capsular serotypes have revealed distinct virulence potential. During periodontitis, P. gingivalis is detected locally in periodontal tissues and systemically in amniotic liquid, being associated with pre-term new-born. Recently, P. gingivalis has been also detected in IV ventricle brain in Alzheimer’s disease individuals, suggestive of a potential role in its pathogenesis. Thus, this study aimed to determine the differential migratory capacity of P. gingivalis serotypes towards hippocampus and/or cerebrospinal fluid (CSF) in periodontitis-infected rats.
Methods: Thirty male Sprague-Dawley rats were inoculated at days 1 and 7 with 1x1010 CFU/mL of the different P. gingivalis serotypes. Sham-infected rats were used as controls. Samples of periodontal lesions, serum, CSF, and hippocampus were obtained and the P. gingivalis 16S rRNA detection was quantified by qPCR. In addition, the RgpA and Kgp DNA expression levels were determined by qPCR and the RgpA protein levels by flow cytometry.
Results: Thirty male Sprague-Dawley rats were inoculated at days 1 and 7 with 1x1010 CFU/mL of the different P. gingivalis serotypes. Sham-infected rats were used as controls. Samples of periodontal lesions, serum, CSF, and hippocampus were obtained and the P. gingivalis 16S rRNA detection was quantified by qPCR. In addition, the RgpA and Kgp DNA expression levels were determined by qPCR and the RgpA protein levels by flow cytometry.
Conclusions: P. gingivalis is detectable in the peripheral blood circulation, hippocampus and CSF in periodontitis-infected rats independently of the inoculated P. gingivalis strain.
Methods: Thirty male Sprague-Dawley rats were inoculated at days 1 and 7 with 1x1010 CFU/mL of the different P. gingivalis serotypes. Sham-infected rats were used as controls. Samples of periodontal lesions, serum, CSF, and hippocampus were obtained and the P. gingivalis 16S rRNA detection was quantified by qPCR. In addition, the RgpA and Kgp DNA expression levels were determined by qPCR and the RgpA protein levels by flow cytometry.
Results: Thirty male Sprague-Dawley rats were inoculated at days 1 and 7 with 1x1010 CFU/mL of the different P. gingivalis serotypes. Sham-infected rats were used as controls. Samples of periodontal lesions, serum, CSF, and hippocampus were obtained and the P. gingivalis 16S rRNA detection was quantified by qPCR. In addition, the RgpA and Kgp DNA expression levels were determined by qPCR and the RgpA protein levels by flow cytometry.
Conclusions: P. gingivalis is detectable in the peripheral blood circulation, hippocampus and CSF in periodontitis-infected rats independently of the inoculated P. gingivalis strain.