3D Structural Variation of L-PRF Under Different Centrifugation Protoclols

Objectives: To describe and compare the fibrin network architecture, presence and morphology of the leukocytes in the L-PRF under three different centrigutaion protocols.
Methods: Twelve blood samples were collected from 6 voluntary dentistry students between 20 and 25 years old, with no medical history, recent aspirin intake or any condition that affects the coagulation process. This was approved by the ethics committee of the Universidad Austral de Chile in June 8th 2018. These samples were separated equally into three groups: (1) 200G x 12 minutes, (2) 400G x 12 minutes and (3) 600g x 12 minutes (3). From each group, two samples were immersed in 10% formaldehyde for the optical microscope, stained with Masson and Giemsa stains respectively. The other two in 2.5% glutaraldehyde for scanning electron microscopy.
Results: Under the optical microscope, the distribution and cellular architecture of the leukocytes were observed and compared among groups, and the density of the fibrin matrix partially, with leukocytes present in all groups, but the distribuiton and quantity in each group was slightly different, being group (2) the most homogeneous. In the scanning microscope analysis the results shown in the optical microscope were confirmed, and also analyling the quality of the fibrin matrix and morhpological evaluation of cells.The fibrin matrix was dense in all groups, with leukocyte presence, but group (2) was the one with the best quality, quantity and distribution overall.
Conclusions: We can conclude that the 400g x 12 minutes protocol, with the centrifugue we used, shows the best results overall compared with the other two groups, but we must consider that our evaluation was only visual, with one centrifugue brand. To further research the L-PRF quality and ideal protocol, both visual and growth factor measurement should be done, with the same centrifugue and different preparation protocols.