IADR Abstract Archives
Adhesion of Streptococcus mutans on Ceramic with Different Polishing Protocols
Objectives: The aim of this study was to evaluate in vitro the influence of five different polishing protocols on lithium disilicate ceramic on the adhesion of Streptococcus mutans biofilm.
Methods: Fifty specimens of pre-sintered lithium disilicate (IPS e.max CAD) with dimensions of 5 x 5 x 1.2 mm were fabricated and divided into 5 groups: G1- positive control (Glaze Group – treatment just with glaze); G2 (Glaze Group + Wear + Glaze) – wear simulating occlusal adjustment with diamond tip and new glaze; G3 (Wear group - negative control) – wear; G4 (Ceramisté Wear Group) - wear and polishing with Ceramisté Polishing Kit (Shofu); G5 (Optrafine Wear Group) - wear and polishing with Optrafine Polishing Kit (Ivoclar). In order to observe the smoothness surface obtained after the polishing protocols of the specimens, surface roughness (Ra - µm) was evaluated. Biofilms of S. mutans were formed on the surfaces of the samples. For biofilm quantification, the number of cultured cells was evaluated by counting colony forming units (CFUs). The data were submitted to statistical analysis (one-way ANOVA, followed by Tukey's test, p≤0.05).
Results: There was a difference in surface roughness of all groups in relation to G3 (Negative control; p≤0.05), presenting an average Ra of 1.68µm. There was no statistically significant difference between groups that were polished (G4 - 1.32µm and G5 - 1.06µm). The lowest mean roughness values were those of group G1 (positive control; 0.4µm). There was a difference in Log values (CFU/mL) only between the G3 group and the glaze groups (G1 and G2, p≤0.05). The highest adhesion of S. mutans occurred in group G3 (4.53 Log).
Conclusions: The best polishing protocol of lithium disilicate ceramics after wear is glazing on surface, presenting the lowest values of roughness and CFUs.
Methods: Fifty specimens of pre-sintered lithium disilicate (IPS e.max CAD) with dimensions of 5 x 5 x 1.2 mm were fabricated and divided into 5 groups: G1- positive control (Glaze Group – treatment just with glaze); G2 (Glaze Group + Wear + Glaze) – wear simulating occlusal adjustment with diamond tip and new glaze; G3 (Wear group - negative control) – wear; G4 (Ceramisté Wear Group) - wear and polishing with Ceramisté Polishing Kit (Shofu); G5 (Optrafine Wear Group) - wear and polishing with Optrafine Polishing Kit (Ivoclar). In order to observe the smoothness surface obtained after the polishing protocols of the specimens, surface roughness (Ra - µm) was evaluated. Biofilms of S. mutans were formed on the surfaces of the samples. For biofilm quantification, the number of cultured cells was evaluated by counting colony forming units (CFUs). The data were submitted to statistical analysis (one-way ANOVA, followed by Tukey's test, p≤0.05).
Results: There was a difference in surface roughness of all groups in relation to G3 (Negative control; p≤0.05), presenting an average Ra of 1.68µm. There was no statistically significant difference between groups that were polished (G4 - 1.32µm and G5 - 1.06µm). The lowest mean roughness values were those of group G1 (positive control; 0.4µm). There was a difference in Log values (CFU/mL) only between the G3 group and the glaze groups (G1 and G2, p≤0.05). The highest adhesion of S. mutans occurred in group G3 (4.53 Log).
Conclusions: The best polishing protocol of lithium disilicate ceramics after wear is glazing on surface, presenting the lowest values of roughness and CFUs.