IADR Abstract Archives
Apoptotic Effect of Three Universal Dental Adhesives in SaOS-2 Cells
Objectives: To evaluate the potential apoptosis-mediated cytotoxicity of three universal dental adhesives in the SaOS-2 osteoblast-type cell line
Methods: Three universal dental adhesives (Universal Single Bond - 3M ESPE; Universal Ambar - FGM; Prime & Bond Universal - Dentsply) were evaluated at a concentration of 0.1% v/v in the Saos-2 cell line. Three apoptosis indicators were evaluated after 2-4-6h incubation with the adhesives: phosphatidylserine and propidium iodide detection through flow cytometry, detection of cleaved Caspase-3 by immunoblot and evaluation of nuclear morphology through fluorescence microscopy. The cytotoxicity of the adhesives was determined after 24h incubation by MTT assay, in the absence or presence of the Z-VAD-FMK apoptosis inhibitor.
Results: A higher percentage of cells in early apoptosis was observed by flow cytometry with the Single Bond adhesive, while the Ambar and Prime & Bond adhesives showed a significant increase in death through necrosis. Only the Single Bond adhesive displayed a significant increase of cleaved Caspase-3 compared to the control. The immunofluorescence imaging evaluation showed apoptosis-compatible cell nucleus for the Single Bond adhesive, while Ambar and Prime & Bond adhesives presented necrotic-like nucleus. The MTT test indicated that the three adhesives significantly reduced cell viability: Single Bond (47,9%), Ambar and Prime & Bond (0%); The Z-VAD-PMK apoptosis inhibitor combined with the Single Bond adhesive significantly increased viability to 79,8%.
Conclusions: The evaluated adhesives induce cytotoxicity in Saos-2 cells, with Single Bond being the least aggressive. The results suggest that the Single Bond adhesive mainly causes cell death by apoptosis, while Ambar and Prime & Bond show a tendency to cell death by necrosis
Methods: Three universal dental adhesives (Universal Single Bond - 3M ESPE; Universal Ambar - FGM; Prime & Bond Universal - Dentsply) were evaluated at a concentration of 0.1% v/v in the Saos-2 cell line. Three apoptosis indicators were evaluated after 2-4-6h incubation with the adhesives: phosphatidylserine and propidium iodide detection through flow cytometry, detection of cleaved Caspase-3 by immunoblot and evaluation of nuclear morphology through fluorescence microscopy. The cytotoxicity of the adhesives was determined after 24h incubation by MTT assay, in the absence or presence of the Z-VAD-FMK apoptosis inhibitor.
Results: A higher percentage of cells in early apoptosis was observed by flow cytometry with the Single Bond adhesive, while the Ambar and Prime & Bond adhesives showed a significant increase in death through necrosis. Only the Single Bond adhesive displayed a significant increase of cleaved Caspase-3 compared to the control. The immunofluorescence imaging evaluation showed apoptosis-compatible cell nucleus for the Single Bond adhesive, while Ambar and Prime & Bond adhesives presented necrotic-like nucleus. The MTT test indicated that the three adhesives significantly reduced cell viability: Single Bond (47,9%), Ambar and Prime & Bond (0%); The Z-VAD-PMK apoptosis inhibitor combined with the Single Bond adhesive significantly increased viability to 79,8%.
Conclusions: The evaluated adhesives induce cytotoxicity in Saos-2 cells, with Single Bond being the least aggressive. The results suggest that the Single Bond adhesive mainly causes cell death by apoptosis, while Ambar and Prime & Bond show a tendency to cell death by necrosis