IADR Abstract Archives
Association Between Lymphocyte B-Chemotactic Cytokine Levels in Gingival Crevicular Peri-Implant Fluid and Peri-Implant Diagnosis.
Objectives: To explore the levels and association between lymphocyte B-chemotactic cytokines in gingival crevicular peri-implant fluid and the peri-implant diagnosis.
Methods: Patients that were receiving periodontal maintenance therapy for dental implants were recruited at Dental Health Center of the Universidad de la Frontera, Temuco, Chile. The Ethics Committee of the Universidad de la Frontera approved the present study and all participants signed an informed consent. Clinical assessment was performed by a single operator and included complete periodontal and peri-implant diagnosis: gingival health (GH), peri-mucositis (PM) and peri-implantitis (PI), periodontal pocket depth (PPD), bleeding on probing (BOP), suppuration, time of implant placement, bacterial plaque percentage (BPP), periodontal disease history and cigarette smoking. The sample of gingival crevicular peri-implant fluid (GCPIF) was processed by Luminex® for quantification of chemokine C-C motif ligand 20 (CCL-20), proliferation-inducing-ligand (APRIL), B-cell activating factor (BAFF-BLYS), interleukin 23 (IL-23), Receptor activator of nuclear factor kappa-β ligand (RANK-L) and osteoprotegerin (OPG), (pg/ml). Data was analyzed by descriptive statistics, Kruskall-Wallis and Spearman’s correlation tests.
Results: A total of 12 subjects were recruited with 48 implants, of them: 16 implants were diagnosed with GH, 16 PM and 16 PI. From the clinical records: positive history of periodontal disease (p-value=0.014), current periodontal disease (p-value=0.001), BOP (p-value=0.013), BPP (p-value=0.018), PPD (p-value=0.0001) were significantly associated to peri-implant diagnosis. Among the panel of cytokines, CCL20 had a median value of 50.24 (IQR=93.42), APRIL 161.46 (IQR=216.15), BAFF-BLYS 9.7 (IQR=10.262), IL-23 143.65 (IQR=153.575), RANK-L 606.78 (IQR=158.86) and OPG=1701 (IQR=1683.5), pg/ml. However, only APRIL was significantly associated to PM (p-value=0.008) and PI (p-value=0.0008).
Conclusions: This study employed a novel technology for simultaneous cytokine quantification in GCPIF. APRIL, a lymphocyte B-chemoattractant cytokine, was increased in PI and PM, suggesting a mechanism of B-cell activation via RANK-L. Further investigations are required to elucidate this link and assess APRIL as a potential biomarker of tissue destruction in PI and PM.
Methods: Patients that were receiving periodontal maintenance therapy for dental implants were recruited at Dental Health Center of the Universidad de la Frontera, Temuco, Chile. The Ethics Committee of the Universidad de la Frontera approved the present study and all participants signed an informed consent. Clinical assessment was performed by a single operator and included complete periodontal and peri-implant diagnosis: gingival health (GH), peri-mucositis (PM) and peri-implantitis (PI), periodontal pocket depth (PPD), bleeding on probing (BOP), suppuration, time of implant placement, bacterial plaque percentage (BPP), periodontal disease history and cigarette smoking. The sample of gingival crevicular peri-implant fluid (GCPIF) was processed by Luminex® for quantification of chemokine C-C motif ligand 20 (CCL-20), proliferation-inducing-ligand (APRIL), B-cell activating factor (BAFF-BLYS), interleukin 23 (IL-23), Receptor activator of nuclear factor kappa-β ligand (RANK-L) and osteoprotegerin (OPG), (pg/ml). Data was analyzed by descriptive statistics, Kruskall-Wallis and Spearman’s correlation tests.
Results: A total of 12 subjects were recruited with 48 implants, of them: 16 implants were diagnosed with GH, 16 PM and 16 PI. From the clinical records: positive history of periodontal disease (p-value=0.014), current periodontal disease (p-value=0.001), BOP (p-value=0.013), BPP (p-value=0.018), PPD (p-value=0.0001) were significantly associated to peri-implant diagnosis. Among the panel of cytokines, CCL20 had a median value of 50.24 (IQR=93.42), APRIL 161.46 (IQR=216.15), BAFF-BLYS 9.7 (IQR=10.262), IL-23 143.65 (IQR=153.575), RANK-L 606.78 (IQR=158.86) and OPG=1701 (IQR=1683.5), pg/ml. However, only APRIL was significantly associated to PM (p-value=0.008) and PI (p-value=0.0008).
Conclusions: This study employed a novel technology for simultaneous cytokine quantification in GCPIF. APRIL, a lymphocyte B-chemoattractant cytokine, was increased in PI and PM, suggesting a mechanism of B-cell activation via RANK-L. Further investigations are required to elucidate this link and assess APRIL as a potential biomarker of tissue destruction in PI and PM.