IADR Abstract Archives
Influence Of Omega-3 Supplementation On Inflammatory Response Of Endodontic Sealers
Objectives: The aim of this study was to evaluate the influence of omega-3 supplementation on inflammatory response of endodontic sealers.
Methods: Forty-eight Wistar rats were assigned to three sealers groups (Sealapex, AH Plus and Endofill) and control group (empty tubes). These materials were placed in polyethylene tubes and implanted into the dorsal connective tissue of Wistar rats for 5, 15, and 30 days (n = 8). Half of the animals received omega-3 supplementation for 15 days before implantation and until the time of sacrifice. After each experimental time, the tubes and surrounding tissue were removed and processed for histological analysis in hematoxylin and eosin stained. The results were statistically analyzed (p < 0.05).
Results: It was observed a lower inflammatory response in the animals supplemented with omega-3 in the 30-day period (p <0.001). Among the no supplementation groups, there was no difference between the evaluated sealers in any of the analysis periods, however Endofill presented a more intense inflammatory infiltrate compared to the control group in the 30-day period (p = 0.009). Among the supplemented groups there was no statistical difference between the sealers. When comparing each sealer with or without supplementation, the sealers Sealapex (p = 0.005) and Endofill (p = 0.001) showed lower inflammatory infiltrate in the 30-day period.
Conclusions: It was concluded that omega 3 supplementation influences the inflammatory response of Sealapex and Endofill endodontic sealers.
Methods: Forty-eight Wistar rats were assigned to three sealers groups (Sealapex, AH Plus and Endofill) and control group (empty tubes). These materials were placed in polyethylene tubes and implanted into the dorsal connective tissue of Wistar rats for 5, 15, and 30 days (n = 8). Half of the animals received omega-3 supplementation for 15 days before implantation and until the time of sacrifice. After each experimental time, the tubes and surrounding tissue were removed and processed for histological analysis in hematoxylin and eosin stained. The results were statistically analyzed (p < 0.05).
Results: It was observed a lower inflammatory response in the animals supplemented with omega-3 in the 30-day period (p <0.001). Among the no supplementation groups, there was no difference between the evaluated sealers in any of the analysis periods, however Endofill presented a more intense inflammatory infiltrate compared to the control group in the 30-day period (p = 0.009). Among the supplemented groups there was no statistical difference between the sealers. When comparing each sealer with or without supplementation, the sealers Sealapex (p = 0.005) and Endofill (p = 0.001) showed lower inflammatory infiltrate in the 30-day period.
Conclusions: It was concluded that omega 3 supplementation influences the inflammatory response of Sealapex and Endofill endodontic sealers.
Chilean Division Meeting
2019 Chilean Division Meeting (Santiago, Chile)
Santiago, Chile
2019
De Oliveira, Pedro Henrique
( São Paulo State University (UNESP)
, Araçatuba
, São Paulo
, Brazil
)
Castilho Jacinto, Rogério
( São Paulo State University (UNESP)
, Araçatuba
, São Paulo
, Brazil
)
Dezan-junior, Eloi
( UNESP
, Aracatuba
, Brazil
)
Gomes-filho, João
( São Paulo State University (UNESP)
, Araçatuba
, São Paulo
, Brazil
)
Cintra, Luciano
( UNESP-Univ Estadual Paulista
, Araçatuba
, SP
, Brazil
)
Rodrigues, Michael Júnio
( São Paulo State University (UNESP)
, Araçatuba
, São Paulo
, Brazil
)
Da Silva, Cristiane
( São Paulo State University (UNESP)
, Araçatuba
, São Paulo
, Brazil
)
Silva, Leopoldo
( São Paulo State University (UNESP)
, Araçatuba
, São Paulo
, Brazil
)
Camacho, Lariana
( São Paulo State University (UNESP)
, Araçatuba
, São Paulo
, Brazil
)
De Azevedo, Júlia
( São Paulo State University (UNESP)
, Araçatuba
, São Paulo
, Brazil
)
Banci, Henrique
( São Paulo State University (Unesp), School of Dentistry, Araçatuba
, Araçatuba
, São Paulo
, Brazil
)
Tavares, Daniela
( São Paulo State University (UNESP)
, Araçatuba
, São Paulo
, Brazil
)
Sivieri-araújo, Gustavo
( São Paulo State University (UNESP)
, Araçatuba
, São Paulo
, Brazil
)
None
FAPESP (São Paulo Research Foundation) - 2017/24713-7
2019 Chilean Division Meeting (Santiago, Chile)
Santiago, Chile
2019
FAPESP (São Paulo Research Foundation) - 2017/24713-7
Oral Session
Oral Session Presentations
Oral Session Presentations
Scores observed for inflammatory response of endodontic sealers and oral supplementation
*Kruskal-Wallis for all eight groups
**Mann-Whitney for two groups (ômega supplementation versus without ômega supplementation)
Data expressed in form of median.
| Period of analysis | Score | H.E. Staining for Groups | Statistical analysis | |||||||
| Control | Control + Ômega | AH Plus | AH Plus + Ômega | Endofill | Endofill + Ômega | Sealapex | Sealapex+ Ômega | |||
| 5 days | 1 | 0/8 | 0/8 | 0/8 | 0/8 | 0/8 | 0/8 | 0/8 | 0/8 | *Kruskal-Wallis p=0.983 **Mann-Whitney p=0.451 |
| 2 | 2/8 | 2/8 | 1/8 | 2/8 | 1/8 | 2/8 | 2/8 | 3/8 | ||
| 3 | 3/8 | 3/8 | 3/8 | 3/8 | 3/8 | 3/8 | 3/8 | 2/8 | ||
| 4 | 3/8 | 3/8 | 4/8 | 3/8 | 4/8 | 3/8 | 3/8 | 3/8 | ||
| Median | 3,5 | 3,5 | 4 | 3,5 | 4 | 3,5 | 3,5 | 3 | ||
| 15 days | 1 | 1/8 | 0/8 | 0/8 | 0/8 | 0/8 | 0/8 | 0/8 | 0/8 | *Kruskal-Wallis p=0.212 **Mann-Whitney p=0.007 |
| 2 | 1/8 | 5/8 | 2/8 | 4/8 | 1/8 | 4/8 | 2/8 | 5/8 | ||
| 3 | 4/8 | 3/8 | 5/8 | 3/8 | 4/8 | 3/8 | 6/8 | 3/8 | ||
| 4 | 2/8 | 0/8 | 1/8 | 1/8 | 3/8 | 1/8 | 0/8 | 0/8 | ||
| Median | 3 | 2 | 3 | 2 | 3 | 2 | 3 | 2 | ||
| 30 days | 1 | 3/8 | 7/8 | 2/8 | 2/8 | 0/8 | 3/8 | 0/8 | 5/8 | *Kruskal-Wallis p<0.001 **Mann-Whitney p<0.001 |
| 2 | 3/8 | 1/8 | 3/8 | 5/8 | 1/8 | 4/8 | 4/8 | 3/8 | ||
| 3 | 2/8 | 0/8 | 3/8 | 1/8 | 5/8 | 1/8 | 4/8 | 0/8 | ||
| 4 | 0/8 | 0/8 | 0/8 | 0/8 | 2/8 | 0/8 | 0/8 | 0/8 | ||
| Median | 1,5AB | 1A | 2,5ABC | 2AB | 3C | 2AB | 2,5BC | 1A | ||
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