IADR Abstract Archives
Biodentine Generates Cellular Cytotoxicity and Induces Specific Apoptotic Activity
Objectives: The objective of this research work was to evaluate endodontic cement based on tricalcium silicate Biodentine according to its biological properties, specifically its ability to generate cell proliferation. This is why the viability, cell cytotoxicity and the capacity to induce apoptosis by the biomaterial on of human dental pulp cell cultures (HDPCs) were studied.
Methods: HDPCs were obtained through primary cultures. The Biodentine preparation was carried out following the manufacturer's instructions, preparing cement disks (6 x 2 mm) under luminous flux and sterilized by UV radiation. The cytotoxicity and cell viability was measured under the MTT colorimetric method. Besides, the ability to induce apoptosis by Biodentine was performed through Western Blot immureactivity assays for the visualization of three apoptotic proteins; Caspase 3, Caspase 3 clivated and PARP -1. All trials were conducted in three study times 24, 48 and 72 hours, compared with a control group cultured with DMEM.
Results: The results show high cytotoxic activity and low viability by Biodentine (p < 0.05) at 24, 48 and 72 hours of exposure compared to control group. In addition, the cement demonstrates high expressivity of Caspase 3 clivated at 48 and 72 hours (p < 0.05) and PARP-1 at 48 hours (p < 0.05) and 72 hours (p < 0.001) compared to control group.
Conclusions: Biodentine exhibits cytotoxic, low viability and specific apoptotic activity on HDPCs. While this study demonstrates the expression of apoptotic proteins, previous trials have also demonstrated apoptotic activity. This is why the use of this biomaterial should be studied for each particular clinical case, especially as a direct pulp capping agent.
Methods: HDPCs were obtained through primary cultures. The Biodentine preparation was carried out following the manufacturer's instructions, preparing cement disks (6 x 2 mm) under luminous flux and sterilized by UV radiation. The cytotoxicity and cell viability was measured under the MTT colorimetric method. Besides, the ability to induce apoptosis by Biodentine was performed through Western Blot immureactivity assays for the visualization of three apoptotic proteins; Caspase 3, Caspase 3 clivated and PARP -1. All trials were conducted in three study times 24, 48 and 72 hours, compared with a control group cultured with DMEM.
Results: The results show high cytotoxic activity and low viability by Biodentine (p < 0.05) at 24, 48 and 72 hours of exposure compared to control group. In addition, the cement demonstrates high expressivity of Caspase 3 clivated at 48 and 72 hours (p < 0.05) and PARP-1 at 48 hours (p < 0.05) and 72 hours (p < 0.001) compared to control group.
Conclusions: Biodentine exhibits cytotoxic, low viability and specific apoptotic activity on HDPCs. While this study demonstrates the expression of apoptotic proteins, previous trials have also demonstrated apoptotic activity. This is why the use of this biomaterial should be studied for each particular clinical case, especially as a direct pulp capping agent.