IADR Abstract Archives
Comparative analysis of the immunomodulatory properties of human gingival mesenchymal stem cells from healthy subjects, and subjects with clinical diagnosis of chronic periodontitis
Objectives: Compare the immunomodulatory properties of mesenchymal stem cells derived from gingival tissue (GMSC) of healthy subjects and subjects with chronic periodontitis.
Methods: Gingival tissue explants were obtained from subjects with surgical
indication. All donors voluntarily signed an informed consent. The cells were isolated and cultured for 3-4 weeks, characterized and induced to differentiate into osteogenic, chondrogenic and adipogenic lineage, according to the criteria of the International Society of Cell Therapy (ISCT). The immunosuppressive capacity was determined by comparing the effect of GMSC on the proliferation of CD4 and CD8 lymphocytes, the generation of regulatory T cells (Treg), the production of molecules associated with immunosuppression as intercellular adhesion molecule (ICAM) and programmed death-ligand 1 (PDL-1) by activated GMSC, by flow
cytometry. The synthesis and release of interleukins 10, 6 and 8 (IL10, IL6, IL8) were measured through an enzyme-linked immunosorbent assay (ELISA).
Results: All GMSC isolated and characterized from healthy and with periodontitis donors fulfilled the minimum criteria to be considered mesenchymal stem cells according to the ISCT. GMSC derived from an inflamed tissue showed greater inhibition of CD4 lymphocytes (31.2 ± 9.4%, p <0.0001) compared to healthy tissue
cells (48.9 ± 20%; p 0.0290) and inhibition of CD8 lymphocytes was only significant in GMSC derived from tissue with periodontal disease (63.6 ± 17%; p 0.0335) when they were co-cultured at a peripheral blood mononuclear cell (PBMC) : GMSC 1:10 ratio. Additionally, the proliferation of Treg cells and the synthesis of IL-10, IL6, IL8 and immunosuppressive molecules such as ICAM and PDL-1, increased similarly in both types of GMSC.
Conclusions: Periodontitis does not seem to change the phenotypic and functional characteristics of the GMSC, however, GMSC isolated from tissue with periodontitis have a greater immunosuppressive potential on T lymphocytes.
Methods: Gingival tissue explants were obtained from subjects with surgical
indication. All donors voluntarily signed an informed consent. The cells were isolated and cultured for 3-4 weeks, characterized and induced to differentiate into osteogenic, chondrogenic and adipogenic lineage, according to the criteria of the International Society of Cell Therapy (ISCT). The immunosuppressive capacity was determined by comparing the effect of GMSC on the proliferation of CD4 and CD8 lymphocytes, the generation of regulatory T cells (Treg), the production of molecules associated with immunosuppression as intercellular adhesion molecule (ICAM) and programmed death-ligand 1 (PDL-1) by activated GMSC, by flow
cytometry. The synthesis and release of interleukins 10, 6 and 8 (IL10, IL6, IL8) were measured through an enzyme-linked immunosorbent assay (ELISA).
Results: All GMSC isolated and characterized from healthy and with periodontitis donors fulfilled the minimum criteria to be considered mesenchymal stem cells according to the ISCT. GMSC derived from an inflamed tissue showed greater inhibition of CD4 lymphocytes (31.2 ± 9.4%, p <0.0001) compared to healthy tissue
cells (48.9 ± 20%; p 0.0290) and inhibition of CD8 lymphocytes was only significant in GMSC derived from tissue with periodontal disease (63.6 ± 17%; p 0.0335) when they were co-cultured at a peripheral blood mononuclear cell (PBMC) : GMSC 1:10 ratio. Additionally, the proliferation of Treg cells and the synthesis of IL-10, IL6, IL8 and immunosuppressive molecules such as ICAM and PDL-1, increased similarly in both types of GMSC.
Conclusions: Periodontitis does not seem to change the phenotypic and functional characteristics of the GMSC, however, GMSC isolated from tissue with periodontitis have a greater immunosuppressive potential on T lymphocytes.
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