IADR Abstract Archives
Metalloproteinases 8 and 9 and periodontal condition during pregnancy
Objectives: To explore the association between metalloproteinases (MMP) 8 and 9 in gingival crevicular fluid (GCF), with periodontal disease severity and clinical parameters during pregnancy
Methods: A cohort study was done with enrollment of 458 pregnant women. Universidad de los Andes IRB approved the study. A complete periodontal examination was performed at 11-14 weeks of gestation by a single calibrated examiner, including: periodontal probing depth (PPD), clinical attachment loss (CAL), bleeding on probing (BOP), plaque index (PI) and periodontal inflamed surface area (PISA). Additionally, GCF samples were taken. All women were classified according to periodontal severity. The concentration of MMP8 and MMP9 were analyzed by Luminex assay. Data was assessed by descriptive statistics; Mann-Whitney test and correlations were determined using Spearman’s correlation coefficient
Results: Levels of MMP-8 were higher in patients with periodontitis in comparison with healthy/gingivitis pregnancies (p=0,000). Also, MMP-8 (ng/ml) was associated with severe chronic periodontitis (OR: 1,000016, p-value=0,000; 95% CI 1.000007- 1.000025). Both MMPs increased in parallel with the severity of the periodontitis. In severe periodontitis, higher levels of both MMPs (MMP-8, p<0,0003 and MMP-9 p<0,001) were observed. Significant correlation was found between MMP-8 and: BOP (rho:0,34, p=0,000), PISA (rho:0,35, p=0,0000), PPD mean (rho: 0,36, p=0,0000), CAL mean (rho:0,33, p=0,0000) and PI (rho: 0,30, p=0,0000). MMP-9, had a significative difference (p=0,0000), but showed a weak correlation with clinical parameters(rho<0.3).
Conclusions: Levels of MMP-8 in GCF are associated with the severity of periodontitis and clinical inflammatory parameters during pregnancy.
Methods: A cohort study was done with enrollment of 458 pregnant women. Universidad de los Andes IRB approved the study. A complete periodontal examination was performed at 11-14 weeks of gestation by a single calibrated examiner, including: periodontal probing depth (PPD), clinical attachment loss (CAL), bleeding on probing (BOP), plaque index (PI) and periodontal inflamed surface area (PISA). Additionally, GCF samples were taken. All women were classified according to periodontal severity. The concentration of MMP8 and MMP9 were analyzed by Luminex assay. Data was assessed by descriptive statistics; Mann-Whitney test and correlations were determined using Spearman’s correlation coefficient
Results: Levels of MMP-8 were higher in patients with periodontitis in comparison with healthy/gingivitis pregnancies (p=0,000). Also, MMP-8 (ng/ml) was associated with severe chronic periodontitis (OR: 1,000016, p-value=0,000; 95% CI 1.000007- 1.000025). Both MMPs increased in parallel with the severity of the periodontitis. In severe periodontitis, higher levels of both MMPs (MMP-8, p<0,0003 and MMP-9 p<0,001) were observed. Significant correlation was found between MMP-8 and: BOP (rho:0,34, p=0,000), PISA (rho:0,35, p=0,0000), PPD mean (rho: 0,36, p=0,0000), CAL mean (rho:0,33, p=0,0000) and PI (rho: 0,30, p=0,0000). MMP-9, had a significative difference (p=0,0000), but showed a weak correlation with clinical parameters(rho<0.3).
Conclusions: Levels of MMP-8 in GCF are associated with the severity of periodontitis and clinical inflammatory parameters during pregnancy.