IADR Abstract Archives
Histological Analysis Of Different Dentin Removal Methods
Objectives: To compare the efficacy of removal of dentin caries in previously extracted human teeth using the rotational carbide, polymer and papain-based mechanical chemistry method, based on histological analysis of remaining caries thickness observed under optical microscopy.
Methods: Twenty seven extracted human molars and premolars with dentinal caries lesion clinically cavitated were randomly divided into 3 groups (n=9) which were handled according to the caries removal method: (1) Conventional mechanical rotary method with carbide burs (Tungsten Carbide H1, Komet Dental), (2) Mechanical rotary method with polymer burs (Polybur P1, Komet Dental) and; (3) Chemo-mechanical method with enzymatic gel based on papain (Brix 3000, Brix Medical Science). The teeth of all groups were sectioned with a microtome into 5 μm thick cuts. The samples were processed histologically with Arteta staining to evaluate the presence and thickness of remaining caries under the optical microscope. Finally, the Micrometrics® SE Premium program is used to project and measure each sample via a computer screen. Differences between groups were analyzed with ANOVA at p<0.05.
Results: A statistically significant difference (p<0.05) in the values of remaining dentine caries thickness was observed between group 2 (chemo-mechanical method, average 47.43um) with respect to group 1 (Polymer bur, average 100.06um) and group 3 (carbide bur, average 102.27um)
Conclusions: The results collected in this study, within its limitations, suggest that both new methods of caries removal (ie. polymer bur and chemo-mechanical) are suitable for clinical use because they have a capacity to eliminate dentinal caries similar to the conventional method.
Methods: Twenty seven extracted human molars and premolars with dentinal caries lesion clinically cavitated were randomly divided into 3 groups (n=9) which were handled according to the caries removal method: (1) Conventional mechanical rotary method with carbide burs (Tungsten Carbide H1, Komet Dental), (2) Mechanical rotary method with polymer burs (Polybur P1, Komet Dental) and; (3) Chemo-mechanical method with enzymatic gel based on papain (Brix 3000, Brix Medical Science). The teeth of all groups were sectioned with a microtome into 5 μm thick cuts. The samples were processed histologically with Arteta staining to evaluate the presence and thickness of remaining caries under the optical microscope. Finally, the Micrometrics® SE Premium program is used to project and measure each sample via a computer screen. Differences between groups were analyzed with ANOVA at p<0.05.
Results: A statistically significant difference (p<0.05) in the values of remaining dentine caries thickness was observed between group 2 (chemo-mechanical method, average 47.43um) with respect to group 1 (Polymer bur, average 100.06um) and group 3 (carbide bur, average 102.27um)
Conclusions: The results collected in this study, within its limitations, suggest that both new methods of caries removal (ie. polymer bur and chemo-mechanical) are suitable for clinical use because they have a capacity to eliminate dentinal caries similar to the conventional method.