IADR Abstract Archives
Expression Levels Of IL17 And IL22 In Symptomatic Apical Periodontitis.
Objectives: To determine mRNA expression levels of IL-17 and IL-22 in symptomatic apical periodontitis (SAP), asymptomatic apical periodontitis (AAP) and healthy controls.
Methods: Patients consulting at the dental clinic, Faculty of Dentistry Universidad Andrés Bello and Emergency Hospital Public Assistance, Santiago, Chile with clinical diagnosis of SAP (n= 9), AAP (n = 9), and controls corresponding to healthy periodontal ligament (n=3) and indication of tooth extraction were included. Patients with antibiotic or anti inflammatory medication during the last three months and/or having immunologic disease were excluded. mRNA was extracted and expression levels of IL-17 and 22 were determined by quantitative real-time PCR. Quality of mRNA was assessed by Bioanalyzer. Results were analyzed with K. Wallis and Bonferroni tests with the STATA program.
Results: IL-22 was significantly over expressed in AAP compared to SAP and controls (p<0.05). The expression of IL-17 mRNA did not show significant differences between AAP, SAP and controls (p<0.05).
Conclusions: IL-22 was over expressed in AAP versus SAP and controls, whereas IL-17 did not show differences. IL-22 might be involved in apical lesion inactivitys.
Methods: Patients consulting at the dental clinic, Faculty of Dentistry Universidad Andrés Bello and Emergency Hospital Public Assistance, Santiago, Chile with clinical diagnosis of SAP (n= 9), AAP (n = 9), and controls corresponding to healthy periodontal ligament (n=3) and indication of tooth extraction were included. Patients with antibiotic or anti inflammatory medication during the last three months and/or having immunologic disease were excluded. mRNA was extracted and expression levels of IL-17 and 22 were determined by quantitative real-time PCR. Quality of mRNA was assessed by Bioanalyzer. Results were analyzed with K. Wallis and Bonferroni tests with the STATA program.
Results: IL-22 was significantly over expressed in AAP compared to SAP and controls (p<0.05). The expression of IL-17 mRNA did not show significant differences between AAP, SAP and controls (p<0.05).
Conclusions: IL-22 was over expressed in AAP versus SAP and controls, whereas IL-17 did not show differences. IL-22 might be involved in apical lesion inactivitys.