IADR Abstract Archives
Dentifrice containing EGCG remineralizes root dentin in vitro
Objectives: This in vitro study analyzed the effect of fluoride concentration, pH and presence of epigallocathechin-gallate (EGCG) remineralization of carious dentin.
Methods: Bovine root dentin specimens (n=144) were divided into 12 groups according to their surface hardness: 1- placebo dentifrice pH 7; 2 - placebo dentifrice pH 4.5; 3 - placebo dentifrice pH 7 with 0.61% EGCG; 4 - placebo dentifrice pH 4.5 with 0.61% EGCG; 5 - 1100 ppm F dentifrice pH 7 without EGCG; 6 - 1100 ppm F dentifrice pH 7 with 0.61% EGCG; 7 - 1100 ppm F dentifrice pH 4.5 without EGCG; 8 - 1100 ppm F dentifrice pH 4.5 with 0.61% EGCG; 9 - 5000 ppm F dentifrice pH 7 without EGCG;10 - 5000 ppm F dentifrice pH 7 with EGCG 0.61%; G 11 - 5000 ppm F dentifrice pH 4.5 with 0.61% EGCG; 12 - 5000 ppm F dentifrice pH 4.5 without EGCG. The specimens were subjected to a pH cycling model for 7 days (6 h demineralization, 18 h remineralization). Treatment with dentifrices was done before and after remineralization using a brushing machine, toothbrush (Curaprox) and dentifrices tested (2x15 s, 150 g load). Percentage of surface hardness recovery (%SHR) was calculated. Data were analyzed by ANOVA and Bonferroni post hoc test (p<0.05).
Results: ANOVA found a significant difference between the groups (F = 34.396 p <0.0001). The lowest % SHR% was observed for group 2, which differed from all others, except from groups 1 and 4. The highest %SHR was observed for group 11, which differed from the others, except from groups 6, 8, 9, 10 and 12.
Conclusions: The results indicate that for recovery of surface hardness in carious root dentin, the most appropriate treatment is the dentifrice with 1100 ppm F pH 7 containing EGCG.
Financial support: FAPESP (2017/08585-9).
Methods: Bovine root dentin specimens (n=144) were divided into 12 groups according to their surface hardness: 1- placebo dentifrice pH 7; 2 - placebo dentifrice pH 4.5; 3 - placebo dentifrice pH 7 with 0.61% EGCG; 4 - placebo dentifrice pH 4.5 with 0.61% EGCG; 5 - 1100 ppm F dentifrice pH 7 without EGCG; 6 - 1100 ppm F dentifrice pH 7 with 0.61% EGCG; 7 - 1100 ppm F dentifrice pH 4.5 without EGCG; 8 - 1100 ppm F dentifrice pH 4.5 with 0.61% EGCG; 9 - 5000 ppm F dentifrice pH 7 without EGCG;10 - 5000 ppm F dentifrice pH 7 with EGCG 0.61%; G 11 - 5000 ppm F dentifrice pH 4.5 with 0.61% EGCG; 12 - 5000 ppm F dentifrice pH 4.5 without EGCG. The specimens were subjected to a pH cycling model for 7 days (6 h demineralization, 18 h remineralization). Treatment with dentifrices was done before and after remineralization using a brushing machine, toothbrush (Curaprox) and dentifrices tested (2x15 s, 150 g load). Percentage of surface hardness recovery (%SHR) was calculated. Data were analyzed by ANOVA and Bonferroni post hoc test (p<0.05).
Results: ANOVA found a significant difference between the groups (F = 34.396 p <0.0001). The lowest % SHR% was observed for group 2, which differed from all others, except from groups 1 and 4. The highest %SHR was observed for group 11, which differed from the others, except from groups 6, 8, 9, 10 and 12.
Conclusions: The results indicate that for recovery of surface hardness in carious root dentin, the most appropriate treatment is the dentifrice with 1100 ppm F pH 7 containing EGCG.
Financial support: FAPESP (2017/08585-9).