IADR Abstract Archives
Histological validation of fibrocartilage MRI detection in TMJ
Objectives: The Objective of this study was to validate SPGR MRI (WATS T1) technique to detect the fibrocartilaginous layer of the porcine mandible condyle ex vivo and to compare the SPGR MRI technique with conventional MRI protocol to detect the fibrocartilage layer in TMJ.
Methods: Methods: Two young Yorkshire pig heads, nine months old, obtained from a local slaughterhouse were studied. In both heads, the right condyle fibrocartilages layer was surgically removed, while the left TMJ remains untouched. The two heads were examined using bilateral MRI (Phillips Intera 1,5T, Sense Flex S Dual Coil, Eindhoven, Nederland) in the following sequences; sagittal DP weighted (TR1500; TE20), T2 weighted (TR2000; TE100) and WATS T1 (TR30; TE4.4) all in oblique axis of the mandible condyle at closed mouth. Subsequently, the TMJs were extracted, submerged in buffered 10% formalin solution at room temperature and sent to the pathological anatomy service for the histological report of the condyles. All 4 samples were decalcified by Osteosoft (EDTHA pH 7.0) until suitable for inclusion (Poth Hille Paraffin Wax Plus) and staining (Hematoxilin-Eosin, and Toluidin Blue Cancer Diagnostic).
Results:
Results: The porcine study showed that WATS T1 images clearly detect the presence or absence of fibrocartilaginous layer in the mandibular condyle. WATS T1 sequence allowed the largest display of fibrocartilage, compared with the other sequences. Mean fibrocartilage ROI signal intensity was 74.07(±14.7)IU.
Conclusions: Conclusions: The ex vivo porcine model proved that WATS T1 sequence can clearly detect fibrocartilage layers of the TMJ, confirmed by histological analysis. The WATS T1 is a reliable sequence to detect the TMJ fibrocartilage layer in the mandibular condyle.
Methods: Methods: Two young Yorkshire pig heads, nine months old, obtained from a local slaughterhouse were studied. In both heads, the right condyle fibrocartilages layer was surgically removed, while the left TMJ remains untouched. The two heads were examined using bilateral MRI (Phillips Intera 1,5T, Sense Flex S Dual Coil, Eindhoven, Nederland) in the following sequences; sagittal DP weighted (TR1500; TE20), T2 weighted (TR2000; TE100) and WATS T1 (TR30; TE4.4) all in oblique axis of the mandible condyle at closed mouth. Subsequently, the TMJs were extracted, submerged in buffered 10% formalin solution at room temperature and sent to the pathological anatomy service for the histological report of the condyles. All 4 samples were decalcified by Osteosoft (EDTHA pH 7.0) until suitable for inclusion (Poth Hille Paraffin Wax Plus) and staining (Hematoxilin-Eosin, and Toluidin Blue Cancer Diagnostic).
Results:
Results: The porcine study showed that WATS T1 images clearly detect the presence or absence of fibrocartilaginous layer in the mandibular condyle. WATS T1 sequence allowed the largest display of fibrocartilage, compared with the other sequences. Mean fibrocartilage ROI signal intensity was 74.07(±14.7)IU.
Conclusions: Conclusions: The ex vivo porcine model proved that WATS T1 sequence can clearly detect fibrocartilage layers of the TMJ, confirmed by histological analysis. The WATS T1 is a reliable sequence to detect the TMJ fibrocartilage layer in the mandibular condyle.