IADR Abstract Archives
Local inflammatory response in symptomatic and asymptomatic chronic apical lesions
Objectives:
Objective: Apical periodontitis is characterized by inflammatory bone resorption with formation of an apical lesion, in which tumor necrosis factor (TNF) α, interleukin (IL) -1β, IL-8 and matrix metalloproteinase (MMP) -8 Play a central role. A difference between symptomatic apical periodontitis (SAP) and asymptomatic apical periodontitis (AAP) is described as the first active and the second inactive or chronic. The objective was to determine the levels and association of proinflammatory cytokines and MMP-8 in SAP, AAP and healthy periodontal ligament (HL).
Methods: Methods: Apical lesions of patients with indication of dental extraction and clinical diagnosis of asymptomatic apical periodontitis (n = 15), symptomatic apical periodontitis (n = 11) and healthy periodontal ligaments (n = 5) were included in the dental SAPU of CESFAM Dr Stegger, Cerro Navia. Patients with chronic immune or inflammatory diseases and antibiotic or anti-inflammatory treatment were excluded. The samples were homogenized and the levels of IL-1β, IL-8, TNF-α and MMP-8 were determined. In addition, we recorded age, sex, educational level, smoking habit and periodontal diseases. Data were analyzed using the STATA program and statistical significance was considered if p <0.05.
Results: Results: Significantly higher levels of IL-1β, IL-8 and MMP-8 were found in PAA and PAS compared to HL and positively correlated (p <0.05); However, no statistically significant differences were found between AAP and SAP. TNF-α was found at low levels and there were no statistically significant differences between groups. In addition, the presence of marginal chronic periodontitis was significantly associated with the presence of periapical lesions.
Conclusions: Conclusions: Periapical lesions are associated with a local proinflammatory state, however, no differences were observed between their symptomatic and asymptomatic forms. Although it is proposed that symptomatic lesions represent an active state of the disease, this is not supported by the present results.
Objective: Apical periodontitis is characterized by inflammatory bone resorption with formation of an apical lesion, in which tumor necrosis factor (TNF) α, interleukin (IL) -1β, IL-8 and matrix metalloproteinase (MMP) -8 Play a central role. A difference between symptomatic apical periodontitis (SAP) and asymptomatic apical periodontitis (AAP) is described as the first active and the second inactive or chronic. The objective was to determine the levels and association of proinflammatory cytokines and MMP-8 in SAP, AAP and healthy periodontal ligament (HL).
Methods: Methods: Apical lesions of patients with indication of dental extraction and clinical diagnosis of asymptomatic apical periodontitis (n = 15), symptomatic apical periodontitis (n = 11) and healthy periodontal ligaments (n = 5) were included in the dental SAPU of CESFAM Dr Stegger, Cerro Navia. Patients with chronic immune or inflammatory diseases and antibiotic or anti-inflammatory treatment were excluded. The samples were homogenized and the levels of IL-1β, IL-8, TNF-α and MMP-8 were determined. In addition, we recorded age, sex, educational level, smoking habit and periodontal diseases. Data were analyzed using the STATA program and statistical significance was considered if p <0.05.
Results: Results: Significantly higher levels of IL-1β, IL-8 and MMP-8 were found in PAA and PAS compared to HL and positively correlated (p <0.05); However, no statistically significant differences were found between AAP and SAP. TNF-α was found at low levels and there were no statistically significant differences between groups. In addition, the presence of marginal chronic periodontitis was significantly associated with the presence of periapical lesions.
Conclusions: Conclusions: Periapical lesions are associated with a local proinflammatory state, however, no differences were observed between their symptomatic and asymptomatic forms. Although it is proposed that symptomatic lesions represent an active state of the disease, this is not supported by the present results.