Effect of Malva Sylvestris on the inflammatory response in fibroblasts

The objective of this study was to evaluate the anti-inflammatory effect of Malva sylvestris in periodontitis using cell culture protocols fibroblasts. For the methods realization of biological assays, cells were maintained in DMEM with L-glutamine, supplemented with 10% fetal bovine serum and 1% gentamicin. The in vitro cytotoxicity was evaluated by exposure of 3T3 cells to different concentrations (0.1 to 10 mg / ml) of the hydroalcoholic extract of M. sylvestris for 24 h using the MTT test (bromide [3 - (4,5-dimethyl (thiazole-yl) -3,5-diphenyl] tetrazolium.) cell suspension with 105 cells / mL was distributed in culture dishes with 96 wells which were incubated at 37 ° C for periods of time. The cultured fibroblasts were stimulated with LPS (1 microg / mL) for 24 hours. The amount of nitric oxide generated after sensitization with LPS was done by measuring concentrations of nitric oxide through Griess reagent. hydroalcoholic extract of M. sylvestris were incubated with LPS to assess its effects on nitric oxide production. The results showed that the hydroalcoholic extract of M. sylvestris shows no cytotoxic effect at concentrations tested (p> 0, 05), as well as reduce the production of nitric oxide with maximum inhibition of 62% (p <0.05). Conclusion: Our results indicate the absence of cytotoxic response of the compound in 3T3 fibroblasts as well as potentially reducing production of an important inflammatory mediator , nitric oxide.