Métodos: Se realizó un ensayo clínico randomizado y se incluyeron 16 pacientes con periodontitis crónica moderada a severa (PC) y 8 controles periodontalmente sanos. Los sujetos enfermos se dividieron aleatoriamente en 2 grupos que recibieron terapia periodontal convencional o terapia periodontal convencional más ozonoterapia. Se tomaron muestras de FGC basales y 7 días post-tratamiento en ambos grupos y en los controles. Las muestras fueron eluidas y se midió concentración de proteínas totales e ICTP mediante ensayos comerciales BCA y EIA, respectivamente. Los datos fueron analizados con el programa estadístico Stata V11.1. Se determinó significancia cuando p<0.05.
Resultados: En la medición basal, la CPT fue significativamente mayor en los pacientes con PC versus sanos (p<0,0001). La CPT disminuyó en el grupo de tratamiento experimental (p<0,05), mientras que por el contrario, en el grupo con terapia convencional la CPT aumentó en respuesta al tratamiento (p<0,05). ICTP no demostró variaciones significativas entre los grupos de estudio. En medición basal de PC se observó una correlación significativa entre niveles de ICTP y CPT (r=0,4, p=0,01), no así en sujetos sanos.
Conclusiones: La terapia periodontal combinada con ozono se asocia con reducciones en los niveles de proteínas totales en el FGC a diferencia de la terapia convencional que se asocia con un aumento. Se requiere evaluar los parámetros clínicos para determinar si los cambios en FGC se asocian con una respuesta reparativa o inflamatoria.
Because of its anti-inflammatory and antibacterial properties, ozone therapy has been proposed as adjuvant to conventional periodontal therapy. Carboxy-terminal telopeptide of collagen type I (ICTP) reflects collagen I catabolism and therefore, the loss of periodontal support, particularly of alveolar bone. Objective: To determine the levels of ICTP and total protein concentration (CPT) in gingival crevicular fluid (GCF) in patients undergoing non-surgical periodontal therapy combined or not with ozone therapy and healthy subjects.
Methods: A randomized clinical trial was conducted, including 16 patients with moderate to severe chronic periodontitis (CP) and 8 periodontally healthy controls. Diseased subjects were randomly divided into 2 groups: one received conventional periodontal therapy and the other received conventional periodontal therapy plus ozone. GCF samples were taken at baseline, 7 days post-treatment and also from healthy controls. The samples were eluted and total protein concentration and ICTP were measured by BCA and EIA commercial assays, respectively. Data were analyzed with Stata statistical software V11.1. Significance was determined at p <0.05.
Results: CPT was significantly higher in patients with CP at baseline versus healthy subjects (p <0.0001). After treatment, CPT decreased in the experimental group (p <0.05); conversely, CPT increased with conventional periodontal treatment (p <0.05). ICTP showed no significant variations between groups. A positive correlation was found between ICTP levels and CPT (r = 0.4, p = 0.01) in CP at baseline, but not in healthy subjects.
Conclusions: Periodontal therapy combined with ozone is associated with reductions in GCF total protein levels, whereas conventional therapy associates with TPC increases. Evaluation of clinical parameters is required to determine whether changes in GCF are associated with reparative or inflammatory response.