IADR Abstract Archives

ANTIMICROBIAL EFFECTS OF A BOLDO EXTRACT ON ORAL BACTERIA CULTURES

Aim: The aim of this study was to evaluate the effect of a Peumus Boldus (Boldo) ethanolic (70%) extract on oral bacteria cultures in-vitro. Materials and methods:  Dried and fresh Boldo leaves were macerated for 6 days in 70% EtOH, filtered and mounted on a Rotavapor until complete evaporation of the solvent. Extracts were then dissolved in DMSO to a final concentration of 4 µg/ml. Samples of oral bacteria were obtained from healthy volunteers by means of a sterile cotton swab in inner cheek and dental surfaces, and platted on LB agar plates. After 24 hours, individual colonies were selected, cultured in LB broth and adjusted to OD=0.1. Aliquots of 100 µl were then dissolved in 900 µl LB containing 15 µl of the Boldo extract. After 5, 15 and 25 minutes of incubation, 100 µl of the suspension was removed and washed in PBS. The samples were centrifuged, the supernatant was removed and the pellet was reincorporated into 1 ml of LB broth. After cultivating for 18 hours, the final OD was measured. DMSO and LB were used as controls, and the assay was run in triplicate. T-student statistical test was applied using Origin 6.1, considering statistical difference at the p<0.05 level. Results are shown as the OD means ± SD. Results:  After five minutes of incorporating the extract, a reduction of the OD was observed (0.11 ± 0.02) in comparison to the control (0.20 ± 0.005) and DMSO (0.16 ± 0.01). This reduction was only significant for the dry-leaf extract (p<0.05).  Conclusion:  The ethanolic Peumus Boldus dry-leaf extract reduced the relative bacterial load when applied for 5 minutes on oral bacteria cultures in-vitro. This suggests that Boldo could be used as a potential antiseptic mouthwash.

 

Chilean Division Meeting
2010 Chilean Division Meeting (Valdivia, Chile)
Valdivia, Chile
2010
193
2010 Accepted Abstracts
  • Aguayo Sebastian,
  • Marro Maria Francisca,
  • Aguayo Luis,
  • 2010 Accepted Abstracts