MMP-8, TIMP-1 AND MYELOPEROXIDASE AS BIOMARKERS OF PERIODONTAL DISEASES

Our aim is to determine the usefulness of MMP-8, MPO, TIMP-1 and their association to identify subjets with periodontal diseases. Materials and methods:  Periodontally healthy subjects (n=20), and patients with gingivitis (n=19) and moderate to severe chronic periodontitis (n=18) were enrolled and gingival crevicular fluid (GCF) samples were obtained for 30s. After elution, MMP-8 levels were determined by time-resolved immunofluorometric assay (IFMA) and ELISA, and MPO and TIMP-1 levels, by ELISA. Results were expressed as ng/mL and analyzed with StataV10 software using Kruskal-Wallis test and Spearman`s correlation. Results:  MMP-8, MPO and TIMP-1 levels increased progressively from healthy, gingivitis and periodontitis subjects, with significant differences among all group comparisons. TIMP-1 was almost undetectable in subjets without periodontitis and MMP-8/TIMP-1 ratio was not significant between groups. A correlation between MMP-8 and MPO was present and augmented in line with the severity of periodontal destruction from healthy and gingivitis to periodontitis subjects (r=0.59, p=0.06; r=0.64, p=0.0029; r=0.82, p<0,001, respectively). Conclusion:  MMP-8 and MPO increase in line with periodontal destruction severity. Determination of the levels of MMP-8, MPO and TIMP-1 might be useful as adjuvant for clinical diagnosis of periodontal diseases. The association between MMP-8/MPO/TIMP-1 could reflect MMP-8 activity and thereby, periodontal supporting tissue loss.