Aim: Evaluate the formation of tissue like bone from mouse pulp by the application of a new method of culture in vivo in the parotid area.
Materials and methods: We used 60 male ICR / jcl mice for pulp extraction of unerupted maxillary first molars (PD). 60 ICR / jcl adult (30gr) mice were used as receptors, dividing into two equal groups according to graft: EXPERIMENTAL (PD collagen) and CONTROL (PD without collagen). The surgical procedure for placement of the graft was performed according to the methods developed by the authors. The specimens were reviewed at 15 and 60 days with clinical photographs, histologic sections (HE, Masson trichrome and TRAP) and scanning electron microscopy (SEM).
Results: The two groups retained 100% vascularized graft at 60 days. Hardness impingement was observed in both groups after 15 days. Histological analysis showed that both, the experimental group and control group were similar to formation of bone tissue after 15 days. SEM showed characteristics of bone structures.
Conclusion: Using dental pulp allotransplantation in the parotid area is possible to generate structures like bone tissue.