α-Tocopherol And Selenium Effects Human Gingival And Periodontal Ligament Fibroblasts

Method: Primary cultures of human GF and PDLF were isolated. Four test groups and a control group free of medication was formed. In group E 60µM α-tocopherol, in group ES1, ES2 and ES3 the combination of 60µM α-tocopherol with 5x10^-9M, 10x10^-9M and 50x10^-9M selenium respectively was used. Viability, proliferation, bFGF and col I synthesis from both cell types were evaluated at 24, 48 and 72^nd hours and healing was compared on a new wound healing model at 12, 24, 36, 48 and 72^nd hours.

Result: a-tocopherol alone significantly increased the healing rate of PDLF at 12^th hour and increased bFGF and col I release from GF and PDLF’s at 24, 48 and 72^nd hours. a-tocopherol-selenium combination significantly enhanced the proliferation rate of both cells at 48^th hour, decreased the proliferation of PDLF’s at 72^nd hour, increased the healing rate of GF’s at 12^th hour and PDLF’s at 12 and 48^th hours. bFGF and col I synthesis was also increased in both cell types at 24, 48 and 72^nd hours by a-tocopherol-selenium combination.

Conclusion: a-tocopherol and a-tocopherol/selenium combination is able to accelerate the proliferation rate, wound healing process and increase the synthesis of bFGF and col I both from GF and PDLF’s.