IADR Abstract Archives
Antibacterial Effect of Ag/TiO2/polymer Nanohybrid Coatings of Dental Implant
Objectives:
The prevention of peri-implant inflammation by materials with an antibacterial effect can be necessary when the normal healing process is slowed down and the competition between bacteria and cells is not balanced. The purpose of our experiments was to establish an antibacterial coating which is effective against typical colonizers of the oral cavity in peri-implant inflammations.
Methods:
CP4 titanium discs 1.5 mm thick and 9 mm in diameter were acid-etched and sand-blasted (Denti® System Ltd. Hungary). The following coatings were developed preparing copolymer based nanohybrid layers: i) acrylate based copolymer film; ii) 60% TiO2/40% copolymer; iii) 60% DSTiO2/40% copolymer; iv) 60% AgTiO2/40% copolymer; v) 60% AgDSTiO2/40% copolymer. Dodecyl-sulphate (DS) gave the hydrophobic feature. Surfaces were visualized with SEM (Hitachi S4700, Japan) and AFM (PSIA XE-100, South Korea). Total amount of protein of P. gingivalis was measured by a Micro BCA Protein Assay Kit (Pierce Biotechnology, USA). The number of E. coli colonies was measured by mechanical removing (vortex) of the biofilm from the surface.
Results:
Visualization by SEM showed aggregated particles on sample (iv) and (v). The AFM measurements gave Ra=544±47 nm (mean±SEM) roughness value for the control samples, 33±10 nm for sample (i), 289±44 nm for sample (ii), 293±52 nm for sample (iii) 511±56 nm for sample (iv), 183±16 nm for sample (v). By microbiological testing, a significant decrease (p=0,035) of the number of E. coli colonies on the AgDSTiO2/ copolymer coating (v) was observed compared to the control discs. The total protein of attached P. gingivalis increased on the AgDSTiO2/copolymer layer.
Conclusion:
Hydrophobic coating containing Ag nanoparticles (v) may be an effective antibacterial material against typical colonizers of the oral cavity in peri-implant inflammations, although demonstration of its suitability for the osseointegration process has to be in the focus of further experiments.