The Effect Of C.Hydroxide, Chlorhexidine And MTA In C.Albicans Elimination

Method:

Plates of saboroud dextrose agar (Winlab, Leicestershire, England) were prepared by mixing of calcium hydroxide, 0.2% chlorhexidine ( Hibibos, Bosnalijek, BiH) and MTA powder (ProRoot MTA, Dentsply, Tulsa dental, OK,USA) from 50-0.78 mg/ml by dilution with 10 molten agar at 45⁰C.  

Agar compounds of tested materials were poured into Petri dishes and divided into experimental and control groups. Fresh inoculate of Candida albicans was prepared by growing an overnight culture from stock culture and plated into agar compound of experimental and control groups. All plates were incubated in incubator (Innovens 53, Jouan, France) in 37⁰C. The presence of Candida albicans were recorded after 1h, 24h, 48h and 72h.

Result:

After results elaboration with Kruskal Wallis test, calcium hydroxide didn’t show antifungal effect, compared to 0.2% chlorhexidine and MTA which significantly were more efficient in Candida albicans elimination (p<0.01).

Minimum inhibitory concentration of MTA against Candida albicans was 50 mg/ml

Conclusion:

Based on the results of this study it may be concluded that MTA and chlorhexidine have antifungal effect after 3 days period.