GCF Beta-Crosslaps, Osteocalcin And CRP – Possible Periodontal Disease Biomarkers

Periodontal disease, characterized by a severe chronic inflammation, represents the main cause for tooth loss, affecting almost half of the patients over 50 years old. Currently, bone markers, very useful tools to measure bone remodeling, are assessed in serum and urinary samples. In present, there still  is little information concerning the clinical importance of their gingival crevicular fluid levels in the chronic periodontal disease context. Our experimental study evaluated  beta-crosslaps, osteocalcin and hsCRP levels in gingival crevicular fluid (GCF) from patients with severe chronic periodontities in order to use these parameters in periodontities diagnosis. 

Method:

 We have included in our study 30 patients with severe chronic periodontities  versus 20 controls (clinically healthy volunteers). Probing depth, clinical attachment level, plaque index and papillary bleeding index was recorded. GCF beta-crosslaps, osteocalcin and hsCRP levels were analyzed by enzyme-linked immunosorbent assay (ELISA).

Result:

 Our results revealed statistically significant higher values for GCF beta-crosslaps, osteocalcin and hsCRP  in patients with periodontities  versuscontrols (p < 0.01, p < 0.01, p < 0.005 respectively).  

Conclusion:

 GCF beta-crosslaps, osteocalcin and hsCRP, which were evaluated in this study, are future potential reliable predictors for saliva-based diagnostics in monitoring of bone degradation in periodontal disease .