Method: : Forty-eight maxillary canine teeth were used. Root canals were prepared with the Mtwo rotary system (VDW, Munich, Germany). Five milliliters 5.25 % NaOCl, 5 mL 17% EDTA, and 10 mL saline were used for final irrigation. The canals were filled with CH paste (Kalsin,Turkey). Samples were randomly divided into four experimental groups (n = 10) according to irrigation protocols. Positive and negative control groups (n = 4) were used. In Group 1: 2.5 mL 17% EDTA was used, in Group 2:2.5 mL 5.25 % NaOCl + 2.5 mL 17% EDTA were used, in Group 3:2.5 mL 0.2% chitosan was used and for Group 4: Er;Cr:YSGG (Biolase Technology, Inc, San Clement, CA) laser was used in smear removal mode during 1 minute. The samples were evaluated with scanning electron microscope analysis and scored. Kruskal–Wallis test was used for statistical analysis and Bonferroni method was used to correct set of P values for multiple comparisons.
Result: In the apical thirds, laser group showed inferior mean values and was statistically different from other groups (P < .05); however, there were no significant differences were found among the other groups (P > .05). In the middle and coronal thirds, there were no significant differences among four groups (P > .05). Control groups were statistically different from the experimental groups (P < .05).
Conclusion: According to the findings of the present study , chitosan has similar effect with EDTA and NaOCl on removal of CH. Laser is not convenient for the removal of CH especially in apical part of the root canal. None of these irrigation procedures were sufficient to remove CH completely