Evaluation Of C.albicans/S.mutans Adherence On Provisional Fixed Prosthodontics Materials

Method: The following provisional fixed prosthodontic materials were used in test groups (n=22) TemDent(Schütz, Germany), Imident(Imicryl, Turkey), Tab2000(Kerr, USA), Structur Premium(Voco, Germany), Systemp(Ivoclar Vivadent, Liechtenstein) Acrytemp(Zhermack, Italy). Disc-shaped specimens were prepared 10.0mm in diameter and 2.0mm in height. All specimens were sterilized in ultraviolet light for 24 h. Each group were then divided into 2 subgroups (n=11) according to the microbial suspensions used; Candida albicans and Streptococcus mutans. Specimens were incubated at 37°C with C. albicans and S. mutans for 7 days. One specimen in each group was used to check the sterility (negative control). After incubation, the microbial adherence assay was conducted by using tetrazolium salt 2,3-bis[2-methyloxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide (XTT) assay. Surface roughness of each material was also assessed by atomic force microscope measurements.  Data were statistically analyzed by using ANOVA, Kruskall-Wallis and Tukey HSD tests (p=0.05).

Result: The highest C. albicans adhesion values was observed in Takilon; Imident and Tab 2000 groups while the lowest adhesion was in Systemp group (p<0.05). S. mutans showed maximum adhesion to Structur, Systemp and Acrytemp groups (p<0.05). TemDent group however had the lowest adhesion values for S. mutans (p<0.05). C.albicans adhesion was also found higher than S. mutans to all tested materials (p<0.05).

Conclusion: Within the limitation of this in vitro study, it can be assumed that tested provisional crown and bridge materials may exhibit significant adhesion differences for microbial adhesion. It was also observed that C. albicans was more adhesive to all tested materials than S. mutans.