IADR Abstract Archives

Biomimetic Calcium-Silicate Promotes OFMSC Multi-Lineage Differentiation in Root Canal Microenvironment

Objectives: Orofacial/jaw mesenchymal stem cells (OFMSCs) are postnatal stem cells in alveolar bone with high differentiation capacity. Bioactive calcium-silicate cements are used clinically to promote dental tissue regeneration. We have shown that extracts of two experimental calcium-silicate cements 1) calcium-silicate (wTC) containing di- and tricalcium-silicate, calcium sulphate, and calcium chloride and 2) wTC doped with alpha-tricalcium phosphate (wTC-αTCP) support OFMSC survival. However, it is unclear if calcium-silicate can support in vivo multi-lineage differentiation of OFMSCs in pulp canals. We hypothesized that graft of calcium-silicate and OFMSCs will promote in vivo multi-lineage tissue formation within a tooth root canal.

Methods: Early passage human OFMSCs were expanded in culture. 2 x 106 OFMSCs were mixed with either 40 mg of wTC paste or hydroxyapatite tricalcium phosphate powder (HATCP) as control before being packed into the canal of molar root slices (50 mm) previously demineralized and sterilized with ultraviolet radiation. The graft-root composites were transplanted aseptically into separate subcutaneous pockets of 8 week-old immunocompromised mice. Transplants were harvested at 6 weeks, fixed with 4% paraformaldehyde, decalcified in 10% ethylenediaminetetraacetic acid and embedded in paraffin. Hematoxylin⁄eosin stained sections were prepared for microscopic analysis of regenerated tissues. Unstained sections were evaluated by immunostaining with antibodies to bone sialoprotein (BSP) and dentin matrix protein-1 (DMP-1). Human origin of regenerated tissues was confirmed with human mitochondrial antibody.

Results: OFMSC-wTC graft displayed multi-lineage in vivo differentiation forming bone, adipose tissue and muscle within an abundant fibrous tissue stroma. However, differentiation of OFMSC-HA/TCP graft was limited to bone formation. Tissue regenerated within the root microenvironment using either wTC or HA/TCP as carriers were immunoreactive to BSP, DMP-1 and human mitochondria.

Conclusions: wTC calcium-silicate cement in root canal supports in vivo multi-lineage differentiation of OFMSC. Composite of OFMSC-wTC is a potential donor graft for exploratory endodontic tissue regeneration.

Continental European Division Meeting
2013 Continental European Division Meeting (Florence, Italy)
Florence, Italy
2013
171
Scientific Groups
  • Salat, Pinky  ( University of Pennsylvania School of Dental Medicine, Philadelphia, PA, USA )
  • Anil, Mini  ( University of Pennsylvania, Philadelphia, PA, USA )
  • Feng, Ruoxue  ( University of Pennsylvania, Philadelphia, PA, USA )
  • Gandolfi, Maria Giovanna  ( Laboratory of Biomaterials and Oral Pathology, Department of Biomedical and NeuroMotor Sciences, University of Bologna, Bologna, , Italy )
  • Prati, Carlo  ( University of Bologna, Bologna, N/A, Italy )
  • Akintoye, Sunday  ( University of Pennsylvania School of Dental Medicine, Philadelphia, PA, USA )
    • Poster Session
    Pulp Biology and Regeneration posters
    09/05/2013