IADR Abstract Archives

TNF-a and IFN-gamma gene polymorphisms/haplotypes in oral mucous membrane pemphigoid

Objectives: Cytokine gene polymorphisms have been found to contribute to the pathogenesis of T-cell-related oral diseases including oral lichen planus but nothing is known for chronic, bullous oral/skin diseases characterized by the action of autoantibodies against epithelial or basement membrane zone’s components such as oral mucous membrane pemphigoid-OMMP. The aim of this study was to investigate the prevalence of ΤΝF-a & IFN-gamma’s gene polymorphisms/haplotypes in Greek patients with OMMP.

Methods: Gene polymorphisms/haplotypes of IFN-gamma (+874), TNF-a (-238/-308) were detected in 18 patients compare to 39 healthy individuals. Cytokine typing was performed using the LIFECODES Cytokine-Sequence-Specific-Oligonucleotides (SSOs)-Typing Kit (GEN-PROBE Transplant Diagnostics, Inc) for polymerase chain reaction-(PCR).

Results: No differences were found between OMMP patients and controls for IFN-gamma +874 genotype distributions or A, T alleles frequencies (p=0.190). In contrast, significant differences were observed for the frequencies of TNF-a -308 genotype (G/G) (p=0.007) and A, G alleles frequencies (p=0.004), as well as for TNF-a -238 genotype (G/G) (p=0.0002) and A, G alleles (p=0.003) in TNF-a -238 position.

Conclusions: This study suggests for first time, that specific gene polymorphisms of TNF-a, a mediator of T-cell immune response, may contribute to the pathogenesis of OMMP.         

Continental European Division Meeting
2013 Continental European Division Meeting (Florence, Italy)
Florence, Italy
2013
364
Scientific Groups
  • Andreadis, Dimitrios  ( Aristotle University, Thessaloniki, N/A, Greece )
  • Albanidou-farmaki, Eleni  ( Aristotle University, Thessaloniki, N/A, Greece )
  • Deligiannidis, Aristidis  ( Hippokrateio Hospital, Thessaloniki, N/A, Greece )
  • Katsares, Vassilios  ( Hippokrateio Hospital, Thessaloniki, N/A, Greece )
  • Poulopoulos, Athanasios  ( Aristotle University, Thessaloniki, N/A, Greece )
  • Kolokotronis, Alexandros  ( Aristotle University, Thessaloniki, N/A, Greece )
  • Parapanisiou, Efthimia  ( Hippokrateio Hospital, Thessaloniki, N/A, Greece )
  • Markopoulos, Anastasios  ( Aristotle University, Thessaloniki, N/A, Greece )
    • Poster Session
    Oral Medicine and Pathology-Posters
    09/06/2013