The effect of ozone-application on shear bond strengths of sealers

Objectives: To investigate the effect of ozone treatment on shear bond strength of resin based endodontic sealers to root dentin. Methods: 36 extracted premolars were sectioned buccolingually and 72 root halves were randomly divided into six groups (n = 12). The cut surfaces were ground and pretreated with 5% NaOCl and 17% EDTA solutions for 5 minutes followed by 5 minutes of distilled water irrigation. Three resin based sealers AH Plus (Dentsply), Real Seal (Sybron Endo), Hybrid Root SEAL (Sun Medical) were used. All specimens were then fixed with cold acrylic resin to a plastic cylindrical ring (2 cm in diameter and 2.5 cm height). The first three groups were allocated for ozone treatment (80 %, 120 second, KP probe, Biozonix; Dental high-frequency ozone generator, Germany). Three mm high build-ups with a constant surface area of 3.14 mm2 were created using the sealers and allowed to set (37ºC, 100% humid, 72 hrs). The samples were tested to failure for shear bond strength (1 mm/minute). The data was calculated (MPa) and analyzed Two-way ANOVA, Tukey HSD and Ýndependent-t tests , sig. 0.05). Results: There were statistically significant differences in the shear bond strengths values of the tested groups related to the tested endodontic sealers (P<0.05). AH Plus showed statistically higher bond strength than Real Seal and Hybrid Root SEAL ( P<0.05). Ozon treatment had no effect shear bond strength (p>0.05). Conclusions: Results of this study suggested that ozone treatment did not alter the bond strengths of resin based sealers to dentin. AH Plus showed better adhesive performance than the other sealers both on ozon treated and non-treated root dentin.