Methods:This study, 30 Wistar Albino male rats were used. Rats were divided into 4 groups as Healty(S) saline solution(s), Smelatonin(m), Periodontitis(P)s and Pm. Rat tissue samples were obtained for histomorphometric and histopathologic evaluation. Serum samples were collected in order to determine levels of MDA, SOD, GSH-Px and melatonin. However melatonin levels were evaluated in gingival tissue of the rats.
Results:Ps and Pm groups were similar in histopathological and histomorphometric evaluation. However PMNL density in Pm group was lower than Ps group. Low levels of SOD and GSH-Px, MDA levels were higher in P group when compared with S group. Melatonin was led to a decrease in MDA levels and an increase SOD and GSH-Px levels. Gingival melatonin levels were determined significantly lower in Ps group.
Conclusions: Our study supported that melatonin by virtue of its ability to directly scavenges reactive oxygen species (ROS), indirectly stimulates antioxidant enzymes and decrease of tissue concentration levels of melatonin in inflammation, melatonin may reduce oxidative stress and associated damage in experimental periodontitis.