Bone Healing Around Biphasic HA-(Beta-TCP)/Autologous Platelet Lysates Constructs

Objectives:, Autologous platelet concentrates have been proposed as biological enhancers with bone substitutes in bone reconstructive surgery. HA- (Beta-TCP) (ratio 60/40) (BoneCeramic® Straumann) is a bone substitute frequently used in orofacial applications. The aim of this study was to investigate the effect of autologous platelet lysate (APL), in conjunction with particles of Biphasic HA - (Beta-TCP) ceramic on bone healing. Methods:, Rabbit APL was prepared by freeze thawing from a platelet suspension (109 platelets/ml) according to published methods (Soffer et al., 2006). Critical size defects (10mm in depth and 6mm in diameter) were drilled in lateral femoral condyles of New Zealand rabbits. Defects were filled with particles of biphasic HA - (Beta-TCP) ceramic alone or associated with APL. Animals were sacrificed after 6 weeks. New bone formation and ceramic resorption were evaluated using non-demineralized histology and histomorphometry. Results:, New bone formation was observed around ceramic particles. The mean surface of new bone in the defects filled with biphasic HA - (Beta-TCP) or biphasic HA - (Beta-TCP) +APL constructs was 7, 95 ± 3, 35 mm2 and 7, 55 ± 3.05 mm2 respectively. The mean residual surface of particles in defects filled with biphasic HA -(Beta-TCP) +APL constructs was 6, 54 ± 3, 99 mm2 and biphasic HA - (Beta-TCP) alone was 6, 90 ± 2, 72 mm2. There were no significant difference in bone formation (p=0.629>0.05) and in material resorption (p=0.790>0.05) between Biphasic HA -(Beta-TCP) alone and Biphasic HA -(Beta-TCP) + APL constructs. Conclusions:, The results of this study showed that bioactive components of APL in addition to a Biphasic HA- (Beta-TCP) ceramic did not provide an additional benefit in bone formation and didn't affect the resorption of the material. However, this study confirmed the osteoconduction potential of this bone substitute.