Effect of dexketoprofen on adhesion and growth of osteoblast-like cells

Objectives: The objective of the present study was to set, in vitro, the effect of Dexketoprofen trometanol, a non-steroidal-anti-inflammatory drug (NSAIDs), on proliferation and adhesion in osteoblast-like cells (MG63), because the cyclooxygenase activity and prostaglandins signalling are critical regulators of normal skeletal metabolism and inflammation related to injury or disease. Methods: The adhesion and growth studies were performed with human osteoblast-like cells (MG-63 line) which were cultured as in Dulbecco's Modified Eagle Medium (DMEM) with antibiotic and 10% foetal bovine serum. For the study a suspension containing 1 x 105 cell/ml in DMEM was cultured in 96-well plate in presence of 0 (control group), 1 and 10 µM of Dexketoprofen trometanol (Enantyum®) at 37ºC in CO2 (5%) atmosphere. After 0, 60, 90, 120, 150, 180 min and 24 h the suspension containing unattached cells was removed by pipetting carefully from the surface in order to collect osteoblast-like cells suspension not attached to the surface. Effect of dexketoprofen trometanol on adherence and growth of cultured osteoblasts were detected by MTT assayed. The experiences were carried out threefold and the t Student analysis was followed for the statistical analysis. Results: The treatment with doses of 1 and 10 µM of Dexketoprofen trometanol showed a significant increase on adherence human cultured osteoblast-like cells (MG63) at 60, 90 and 120 min. In all assays, the cell attachment after 120 min was almost the same in the treatment and control group. There was not effect on growth of osteoblast-like cells in the treatment with two doses assays. Conclusions: The dexketoprofen trometanol in therapeutic doses could be the choosing NSAID in those clinical situations which require a regeneration and rapid adherence of bone tissue, like in implantology.