IADR Abstract Archives

Establishment Of The iPS-cells From Human Periodontal Ligament Fibroblast Cells

Objectives: Among the various kinds of fibroblasts existing in the human body, the periodontal ligament fibroblast cells have been suggested as multipotent cells. It is characterized by rapid turnover, a high remodeling capacity and remarkable capacity for renewal and repair. It also differentiates into osteoblasts and cementoblasts. To establish the pluripotent cells form periodontal ligament fibroblast cells can be the great advances in the regenerative therapy in dentistry. Methods: We established the induced pluripotent stem cells from human periodontal ligament fibroblasts by introducing Oct3/4, Sox2, Nanog, Klf4 and Lin28 by retrovirous transfection. Results: Even without the oncogene c-Myc, the reprogramming efficiency was higher than that of any other human fibroblast reported previously. The iPS cells established in this study expressed ES cell markers and formed teratoma in SCID mouse.The periodontal ligament fibroblasts endogenously expressed ES cell markers such as Oct 3/4, Nanog, Klf4 and c-Myc. Conclusions: The periodontal ligament fibroblasts could be an optimal cell source for the iPS cells.
Continental European and Scandinavian Divisions Meeting
2011 Continental European and Scandinavian Divisions Meeting (Budapest, Hungary)
Budapest, Hungary
2011
277
Scientific Groups
  • Nomura, Yoshiaki  ( Tsurumi University, Yokohma, N/A, Japan )
  • Nakamura, Yoshiki  ( Tsurumi University, Yokohma, N/A, Japan )
  • Hanada, Nobuhiro  ( Tsurumi University, Yokohma, N/A, Japan )
  • Ishikawa, Misao  ( Tsurumi University, Yokohma, N/A, Japan )
  • Yashiro, Yuichi  ( Tsurumi University, Yokohma, N/A, Japan )
  • Arai, Chihiro  ( Tsurumi University, Yokohma, N/A, Japan )
  • Sanggarnjanavanich, Seetala  ( Tsurumi University, Yokohma, N/A, Japan )
  • Yamaguchi, Takao  ( Tsurumi University, Yokohma, N/A, Japan )
  • Kakuta, Erika  ( Tsurumi University, Yokohma, N/A, Japan )
  • Ishikawa, Yuichi  ( The Cancer Institute of JFCR, Tokyo, N/A, Japan )
  • Noda, Koji  ( Tsurumi University, Yokohma, N/A, Japan )
    • Poster Session
    Posters: Periodontal Research
    09/02/2011